Applied Biosystems 7300 Installation And Maintenance Manual page 123

Calculate the verification value for the
12.
5000-copy population:
In the Report tab, scroll to a sample in the
a.
5K population.
Apply the values in the Mean Qty and
b.
StdDev Qty columns to the following
equation:
5K value = Mean Qty + 3(StdDev Qty)
Calculate the verification value for the
13.
10000-copy population:
In the Report tab, scroll to a sample in the
a.
10K population.
Apply the values in the Mean Qty and
b.
StdDev Qty columns to the following
equation:
10K value = Mean Qty – 3(StdDev Qty)
Compare the verification values for the 10000-
14.
and 5000-copy populations:
• If the 10K value
5K value (step 12), the 7300/7500/7500
Fast system has passed the installation
verification.
• If the 10K value is not greater than the 5K,
the 7300/7500/7500 Fast system has failed
the installation verification. Troubleshoot
the RNase P verification run. See
"Troubleshooting" on page
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
(step 13) is greater than the
99.
Chapter 7 Verifying the Instrument Performance
Analyzing the TaqMan
a
a
®
RNase P Plate Data
b b
b b
97
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