Preparing For The Calibration - Applied Biosystems 7300 Installation And Maintenance Manual

Preparing for the Calibration

Before performing a pure dye
IMPORTANT!
calibration, you must perform an ROI calibration (see
page 35), a background calibration (see
an optical calibration (7500 systems only, see
page 59).
Wear powder-free
IMPORTANT!
gloves when you handle the pure dye
plates.
Retrieve the spectral calibration kit from the
1.
freezer, then remove all of the pure dye plates.
Return the spectral calibration kit to the freezer.
2.
Allow the pure dye plates to warm to room
3.
temperature (approximately 5 minutes).
Do not remove a pure dye plate
IMPORTANT!
from its packaging until you are ready to run it.
The fluorescent dye in the wells of each pure dye
plate is photosensitive. Prolonged exposure to
light can diminish the fluorescence signal
strength of the plate.
Continue with "Creating a Plate Document for the
Pure Dye Calibration" on page
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
page 49), and
72.
Chapter 6 Performing the Pure Dye Calibration
Preparing for the Calibration
Ambient >00:05:00
Spectral calibration kit
FAM pure dye plate
71
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