Applied Biosystems 7300 Installation And Maintenance Manual page 65

Retrieve the spectral calibration kit from the
2.
freezer, then remove the prepared ROI
calibration plate.
Return the spectral calibration kit to the freezer.
3.
Allow the ROI calibration plate to warm to room
4.
temperature (approximately 5 minutes).
Do not remove an ROI calibration
IMPORTANT!
plate from its packaging until you are ready to
run it. The fluorescent dye in the wells of the
plate is photosensitive. Prolonged exposure to
light can diminish the fluorescence signal
strength of the plate.
Remove the ROI calibration plate from its
5.
packaging. Leave the optical film on the plate.
Do not discard the packaging for the ROI calibration
plate. The plate can be used up to three times if it is
stored in its original packaging sleeve.
Standard plates only: Vortex the plate for
1.
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
Briefly centrifuge the ROI calibration plate in a
2.
centrifuge with a plate adapter (<1500xg).
The plate must be well mixed and
IMPORTANT!
centrifuged.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 4 Performing the Regions of Interest (ROI) Calibration
Ambient
<1500× g
Preparing the ROI Calibration Plate
Spectral calibration kit
ROI calibration plate
>00:05:00
00:00:05
4
39