Table of Contents
Advertisement
Display the data. In the Amplification Plot tab:
5.
Click the upper-left corner of the plate grid
a.
to select all wells.
Select Data Ct vs. Well Position to
b.
display the Ct vs. Well Position plot.
Verify the uniformity of each replicate
6.
population by comparing the groupings of C
values.
Experimental error may cause some wells
Note:
to be amplified insufficiently or not at all. These
wells typically produce C
significantly from the average for the associated
replicate wells. If included in the calculations,
these outlying data (outliers) can result in
erroneous measurements.
If outliers are present, reanalyze the run:
7.
In the plate grid, select the wells that
a.
produced the outlying data.
Select View Well Inspector.
b.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
T
values that differ
T
Chapter 7 Verifying the Instrument Performance
Analyzing the TaqMan
The numbers on the X-axis of the plot correspond to the
wells of the 96-well plate. Beginning at well A1, the wells
are numbered from left-to-right, and top-to-bottom.
1
2
3
4
5
6
13
14
15
16
17
18
25
26
27
28
29
30
37
38
39
40
41
42
49
50
51
52
53
54
61
62
63
64
65
66
73
74
75
76
77
78
85
86
87
88
89
90
7300/7500 system plate
®
RNase P Plate Data
Click
Outliers
7
8
9
10
11
12
19
20
21
22
23
24
31
32
33
34
35
36
43
44
45
46
47
48
55
56
57
58
59
60
67
68
69
70
71
72
79
80
81
82
83
84
91
92
93
94
95
96
Outliers
7
95
Hide quick links:
Advertisement
Table of Contents
Troubleshooting
