Chapter 6 Performing the Pure Dye Calibration
Overview
Custom Dyes
Notes
70
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
The 7300/7500/7500 Fast instrument supports the detection of custom pure dyes (dyes
other than those provided by Applied Biosystems).
Custom dyes must fluoresce within the spectral range measured by the instrument:
• 500 to 650 nm for 7300 systems
• 500 to 700 nm for the 7500/7500 Fast systems
To add custom pure dyes to the Pure Dye set for your instrument, see
Custom Pure Dye Plate" on page
127.
"Creating a