Performing The Roi Calibration - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 4 Performing the Regions of Interest (ROI) Calibration

Performing the ROI Calibration

Performing the ROI Calibration
In the ROI Inspector dialog box:
1.
In the Exposure Time field, enter 2048.
a.
Select Filter A.
b.
Click
2.
Determine if your ROI image is acceptable (the
3.
figures to the right show an unsaturated image
and an oversaturated image). Wells in an
acceptable image:
• Must be as bright as possible without
oversaturating. (When you generate the ROI
calibration on page
displayed if wells are oversaturated).
• Can contain some, but do not have to
contain any, red pixels, which represent
saturation.
Unsaturated
Saturation
Good Acceptable
If your ROI image is acceptable, skip to
4.
If your ROI image is oversaturated, decrease the
Exposure Time by half, then click
. Repeat until you obtain an
acceptable ROI image.
If you cannot obtain an acceptable image, see
"Troubleshooting" on page
Notes
44
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
to generate an ROI image.
45, a warning is
Normal Over
Saturated
Bad
step 5.
48.
Unsaturated ROI image
Over-saturated ROI image
a
b