Applied Biosystems 7300 Installation And Maintenance Manual page 92

Chapter 5 Performing the Background Calibration and Optical Calibration
Troubleshooting
Troubleshooting – Background Calibration
6. Create a new background plate (see
Background Plate" on page
7. Perform a background calibration (see
Background Calibration" on page
8. Click (or select Analysis Extract Background).
9. Repeat step 4 on page 65
position(s).
If the contaminated well positions with the new background
plate are:
– In the same location as you saw in
block is contaminated. Decontaminate the sample block
(see "Decontaminating the Sample Block" on page
– No longer present, the original background plate was
contaminated. You can inspect the original background
plate Make sure there is no particulate matter on the bottom
of the plate or on the cover.
10.If the calibration fails after you use a new background plate or
decontaminate the sample block, perform the following test:
a. Press the tray to open it.
b. Load the black plate tool from the packing kit (or a plate
containing a piece of black paper) into the plate holder.
c. Push the tray back into the instrument.
11.Perform a background calibration (see
Background Calibration" on page
a. Click (or select Analysis Extract Background).
b. Select the Results tab, then select the Spectra tab.
c. Select all wells of the plate document.
12.View the Spectral plot for the peak(s) and choose from the
following:
If the contaminated well is:
– Present, then the optics of your 7300/7500/7500
Fast system may be contaminated. Contact
Applied Biosystems technical support or your service
representative for further assistance.
– Absent, then the sample block is contaminated.
Decontaminate the sample block (see
the Sample Block" on page
Notes
66
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Appendix B, "Creating a
125.)
"Performing the
55).
to examine the contaminated well
step 4, then the sample
"Performing the
55).
"Decontaminating
108).
108).
Black
plate
Contaminated well