Applied Biosystems 7300 Installation And Maintenance Manual page 87

Load the plate in the instrument as described in
8.
"Loading the Plate" on page
Wear powder-free
IMPORTANT!
gloves when you handle the ROI
calibration plate.
Creating a Plate Document for the Optical Calibration Run
Click (or select File New).
1.
Configure the New Document dialog box:
2.
Select Assay Calibration.
a.
Select Container 96-Well Clear.
b.
Select Template Blank Document.
c.
In the Operator field, enter your name.
d.
In the Comments field, enter any additional
e.
information that you want to save to the file
(such as the plate bar code).
In the Plate Name field, enter:
f.
Calibration_<date in DDMMYY format>
For example, the name for a calibration
performed on May 31, 2005 would be:
Calibration_310505.
Click .
g.
In the SDS software, select File Save As.
3.
In the Save As dialog box:
4.
If the Save in field does not display SDS
a.
Documents, navigate to D: drive Applied
Biosystems SDS Documents.
Click .
b.
Continue with "Performing the Optical Calibration"
on page 62.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 5 Performing the Background Calibration and Optical Calibration
Performing the Optical Calibration for the 7500/7500 Fast System
40.
12
Notched
corner
Well A1
7300/7500 System
Standard plate
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
A1
1
A
GR2475
7500
keyed corner
7500 Fast System
Fast plate
a
b
c
d
e
f
g
Select
Should be
SDS Documents
61
5