Overview; Chapter 7 Verifying The Instrument Performance - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 7 Verifying the Instrument Performance

Overview

Overview
Time Required
Materials
Required
®
TaqMan RNase P Instrument
Verification Plate Kit
When to Verify
Instrument
Performance
Purpose of
RNase P Runs
Notes
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Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
You can also use the Real-Time System Setup wizard to perform this procedure.
Note:
To access the wizard, select
System Real-Time System Setup Wizard.
• 7300/7500 systems – ~ 2 hours
• 7500 Fast systems – ~ 40 minutes
®
TaqMan RNase P
Instrument Verification
Plate
Applied Biosystems recommends running a TaqMan
plate:
• When installing the system (you must perform all calibrations in this order: ROI
background optical (7500/7500 Fast systems only) pure dye instrument
verification).
• After moving the instrument to another location.
• As needed to verify the function of the 7300/7500/7500 Fast instrument.
The TaqMan RNase P Instrument Verification Plate run verifies the performance of an
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System. The RNase P plate is
pre-loaded with the reagents necessary for the detection and quantification of genomic
copies of the human RNase P gene (a single-copy gene encoding the RNase moiety of
the RNase P enzyme). Each well contains:
• Reaction mix:
– 7300/7500 systems: 1✕ TaqMan
– 7500 Fast systems: 1✕ TaqMan
• RNase P primers
™
• FAM dye-labeled probe)
• Known concentration of human genomic DNA template
Program Files
Safety
Powder-free
Goggles
Gloves
®
RNase P Instrument Verification
®
Universal PCR Master Mix
®
Fast Universal PCR Master Mix
7300/7500/7500 Fast
GR2170
Centrifuge with plate
adapter