Applied Biosystems 7300 Installation And Maintenance Manual page 73

Press the tray door to move the tray into the
3.
instrument.
Place the calibration plate inside its packaging
4.
sleeve.
7300 systems: Return the packaged plate to the
5.
spectral calibration kit in the freezer.
7500/7500 Fast systems:
– If you will perform background and optical
calibrations (described in the next chapter)
within the next 8 hours, keep the ROI
calibration plate at room temperature in the
packaging. The optical calibration you
perform after background calibration uses the
ROI calibration plate.
– If you be perform background and optical
calibrations on another day, return the
packaged plate to the spectral calibration kit in
the freezer.
Do not discard the ROI calibration plate.
Note:
If you store the plate in its packaging sleeve at
–20 to –25 ° C, you can use the plate up to three
times after you open it.
Continue with "Performing the Background
Calibration and Optical Calibration" on page
After you perform an ROI calibration,
IMPORTANT!
you must also perform a background calibration (see
page 49), an optical calibration (7500 systems only,
see page 59), and pure dye calibrations (see
and instrument verification (see
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 4 Performing the Regions of Interest (ROI) Calibration
49.
page 67),
page 87).
Performing the ROI Calibration
-20 to -
4
47