Analyzing The Background Calibration Data - Applied Biosystems 7300 Installation And Maintenance Manual

In the plate document, select the Results tab,
4.
then select the Spectra tab.
Select all wells of the plate document.
5.
Inspect the raw data for irregular spectral peaks
6.
that exceed the following fluorescent standard
units (FSU):
Filter
A, B, C, D (7300/7500)
E (7500)
If one or more wells produce raw spectra that
exceed the specified FSU, the background plate
or the sample block could contain a fluorescent
contaminant. Determine the source of the
contamination. See "Troubleshooting" on
page 64.
Select File Close.
7.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Chapter 5 Performing the Background Calibration and Optical Calibration
FSU
>72,000
>90,000

Analyzing the Background Calibration Data

a
b
Click to select
all wells
Possible
contamination
5
57