Temperature Calibration Verification Test - Applied Biosystems GeneAmp 2720 User Manual

Temperature calibration verification test

Required
equipment
Setting up the
0.2-mL probe
assembly
Configuring the
2720 thermal
cycler
Thermal Cycler Temperature Verification System For GeneAmp
®
and Veriti System User Guide
Use this test to verify the temperature calibration of your 2720 thermal cycler that
has a 0.2-mL sample block module. The temperature calibration verification test
consists of the following procedures, which you must perform in sequence:
Setting up the 0.2-mL probe assembly
Configuring the 2720 thermal cycler
Running the test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Evaluating the results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Ending the test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PHYSICAL INJURY HAZARD. Hot Surface. Use care when
working around the heated cover and sample block to avoid being burned by hot
components.
This test requires the 0.2-mL Sample Block Module Temperature Verification Kit
(PN 4317939), which includes:
• Cotton swabs
• Light mineral oil
• 2720 temperature verification frame
• 0.2-mL probe assembly
• Model 4500 digital thermometer with a 9V battery installed
1. Lift the lever and open the hinged heated cover.
2. Place the 2720 temperature verification frame on the sample block.
3. Using a cotton swab, coat well A6 with mineral oil.
4. Place the 0.2-mL probe assembly into well A6.
5. Thread the probe wire through the channel in the 2720 temperature verification
frame to prevent damage to the probe and lead wires (see
6. Make sure the probe is connected to the digital thermometer.
7. Pull the lever down to engage the heated cover and 2720 temperature
verification frame.
Seat the probe properly and close the heated cover carefully. If
IMPORTANT!
the probe wire is crushed when you close the cover, the probe can be damaged.
8. Power on the digital thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
1. Power on the thermal cycler. The Main menu opens.
For best results, allow the thermal cycler 15 to 30 minutes to warm up to
ambient temperature before performing the test.
2. Press F4 (Util). The Utilities screen opens.
Chapter 2 Temperature calibration verification test
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PCR Systems 2720, 9600, 9700, 9800,
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Figure 3 on page 8).
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