Performing The Amplification Run - Applied Biosystems 7300 Getting Started Manual

Chapter 5 Generating Amplification Data
STANDARD STANDARD STANDARD

Performing the Amplification Run

Performing the Amplification Run
Select the Instrument tab.
1.
By default, the standard PCR conditions for the
PCR step are displayed.
®
AmpErase UNG Activation
HOLD
2 min @ 50 ° C
Accept default values for:
2.
• Sample volume – 50 µL (20 µL for the Fast
system).
• 9600 Emulation – selected.
The 9600 Emulation feature is not
Note:
available for the 7300 instrument.
Notes
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Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Plus/Minus Assay Getting Started Guide
Times and Temperatures
Initial Steps
®
AmpliTaq Gold DNA
Polymerase Activation
HOLD
10 min @ 95 ° C
PCR (Each of 40 cycles)
Melt
CYCLE
15 sec @ 95 ° C
Anneal/Extend
CYCLE
1 min @ 60 ° C