Preparing An Array Card For Instrument Verification - Applied Biosystems QuantStudio 6 Flex User Manual

Preparing an
array card for
instrument
verification
QuantStudio 6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
b. Centrifuge the reaction plate for 2 minutes at < 1500 rpm.
IMPORTANT! The reaction plate must be well mixed and centrifuged.
c. Confirm that the liquid is at the bottom of each well of the reaction plate. If
not, centrifuge the reaction plate again at a greater rpm and for a longer
period of time.
IMPORTANT! Do not allow the bottom of the plate to become dirty. Fluids
and other contaminants that adhere to the bottom of the reaction plate can
contaminate the sample block and cause an abnormally high background.
IMPORTANT! Perform the following procedure only if you are verifying the
performance of a QuantStudio
Required materials
®
• TaqMan Array Card Sealer
• Centrifuge with centrifuge array card carrier clips and buckets
• Powder-free gloves
• Safety goggles
• Pipettor, 200-µL (with pipette tips)
• RNase P Array Card Instrument Verification Reagents Kit:
®
– TaqMan Array Card
– RNase P Array Card Instrument Verification Reagents Kit, including tubes
with reagent mix for each port (8 tubes total)
Preparing the RNase P array card
IMPORTANT! Wear powder-free gloves while preparing the array card.
1. Remove the Array Card RNase P Kit from the freezer, then allow it to warm to
room temperature (for approximately 15 minutes).
IMPORTANT! You must fill and run the RNase P array card within 60 minutes of
thawing the Array Card RNase P Kit.
2. Remove an array card from its box and place it on a clean, dry surface.
Chapter 2 Calibrate the instrument
Verify the instrument performance
™
7 Flex System with an array card sample block.
2
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