Performing Reverse Transcription - Applied Biosystems 7500 Getting Started Manual

Primer Extended on mRNA
5' 3'
5' cDNA
Reverse
Primer
Oligo d(T) or random hexamer
Synthesis of 1st cDNA strand
5' cDNA
3'
Primer Extended on mRNA
5´ 3´
5´ cDNA
Reverse
Primer
Oligo d(T) or random hexamer
Synthesis of 1st cDNA strand
3´ 5´ cDNA
Generating
Data from
STANDARD TANDAR
RQ Plates
Standard
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide

Performing Reverse Transcription

Introduction
and Example
RQ Experiment
Designing
an RQ
Experiment
Performing
Reverse
Transcription
Generating
Data from
OR
RQ Plates
Fast
Performing
an RQ Study
Chapter 3
See page 18
Isolate total RNA
See page 18
Adjust RNA
concentration
Convert
See page 19
total RNA to cDNA
3
17