Gambro Cobe Spectra Essentials Manual page 22

Preface
Caution: The volume capacity of the disposable tubing set waste
bag limits the number of times you can prime a disposable tubing
set. Prime the Single-Needle ELP and LRS disposable tubing sets
a maximum of two times, and the Dual-Needle ELP, LRS,
AutoPBSC, TPE, RBCX, WBC and Functionally Closed WBC
disposable tubing sets a maximum of three times.
Caution: Before you begin Prime, inspect all tubing especially in
the centrifuge and on the front panel to ensure it is not kinked.
Tubing that is occluded, or partially occluded, can lead to
malfunction or fluid imbalance. Tubing performs without difficulty
as long as the interior diameter of the tubing is larger than the
interior diameter of the needle.
Caution: When using a Functionally Closed WBC disposable
tubing set, you must prime the set with ACD-A to maintain a
functionally closed set.
Caution: Ensure all luer connections are secure.
Caution: The Spectra system does not control the AC infusion
rate in Manual mode. If you are in Manual mode and must change
the inlet pump flow rate or the inlet:AC ratio, exit Manual mode
before making the change.
1-16
Caution: If you decrease the anticoagulant to the patient due to
a citrate reaction during an LPE procedure, you must change the
AC pump flow rate directly since you are in the Manual mode. This
maintains the inlet speed at the same rate and a stable interface.
The inlet:AC ratio increases. Adjust the plasma pump flow rate
when changing the AC pump flow rate using the formula in step 3
under "Setting the Pumps."
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Caution: If using an LRS disposable set to perform a standard
ELP procedure caused an air block to form in the plasma or collect
line, the platelets collected may not be leukoreduced. Count the
final platelet product to determine actual platelet yield and WBC
content.
Caution: If you are using a Functionally Closed WBC disposable
tubing set and the inlet pump flow rate is >50 mL/min, to avoid
ACCESS PRESSURE LOW alarms during the Rinseback operation,
change the inlet pump flow rate to 50 mL/min before starting
Rinseback.
Caution: The channel contains bone marrow fat as well as
RBCs. You may want to wash the RBCs if reinfusion is required.
Caution: The residual RBCs may contain some fat and other
contaminants from the bone marrow harvesting. Consider
washing the RBCs.
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COBE Spectra™ Apheresis System • Essentials Guide