Measuring The G Factor - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)

Measuring the G factor

Include the grating factor, or G factor, whenever polarization measurements are taken.
The G factor corrects for variations in polarization wavelength-response for the emis-
sion optics and detectors. A pre-calculated G factor may be used when all other exper-
imental parameters are constant. In other cases, the system can measure the G factor au-
tomatically before an experimental run. G factors are incorporated into the Anisotropy
scan-type:
1
In the FluorEssence toolbar, click the Experi-
ment Menu button
The Fluorescence Main Experiment
Menu appears.
2
Click the Anisotropy
button.
The Experiment Type window appears.
3
Choose the type of Anisotro-
py experiment, then click the
Next >> button.
The Experiment Setup window opens. To de-
termine polarization at particular excita-
tion/emission wavelength-pairs, choose vs
SinglePoint.
.
5-5
Optimizing Data