Running An Unknown Sample - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)

Running an unknown sample

Often a researcher will scan a sample whose spectral characteristics are unknown. For
optimal spectra, the optimal excitation and emission wavelengths must be found.
The traditional method consists of running an emission scan to find the peak emission
value. Then an excitation scan is run using the determined peak emission value.
1
Find the preliminary emission maximum.
The object of this step is to acquire a preliminary emission scan, based on a
"best-guess" excitation wavelength. Because the fluorescence emission of sam-
ples does not shift with excitation wavelength, the guessed excitation wave-
length yields the emission peak, albeit perhaps at lower intensity.
a
b
c
d
e
Be sure all system components are on, and the FluoroMax
calibrated as explained in Chapter 3.
Click the Experiment Menu button
The Fluorescence Main Exper-
iment Menu appears:
Choose the Spectra button.
The Experiment Type window
appears:
Choose Emission.
Click the Next >> button.
The Experiment Setup window appears:
:
4-23
Data Acquisition
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