Horiba Scientific FluoroMax-4 Operation Manual page 180

® ®
FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
Problem
Poor polarization data
Low polarization ratio
Autopolarizers do not
initialize (they do not
move during initializa-
tion).
Software failure initial-
izing autopolarizers
Cause Possible Remedy
Improper sample Adjust sample concentration.
concentration
Photomultiplier Check that sample signals are in
saturated; slits im- linear region (< 2 × 10
10 μA on R). Reset slits.
properly set
Dirty cuvette Clean the cuvette.
Polarizer misa- Check polarizer alignment.
ligned
Using plastic or cy- Use rectangular, glass or quartz cu-
lindrical cuvette vette.
System misaligned Check system alignment in a gener-
ic layout. Run lamp scan and wa-
ter-Raman scan to check calibra-
tion.
Highly concentrat- Check Ludox
ed standard tration: higher concentrations can
cause inner-filter effect, lowering
ratio.
Improperly set slits Set slits for ~ 1 × 10
Signals much less than this give
excessive contribution from dark
noise, while signals > 2 × 10
are in non-linear region.
System misaligned Check system alignment in generic
layout. Run lamp scan and water-
Raman scan to check calibration.
Wrong instrument Check that a configuration with
configuration is autopolarizers is loaded.
loaded
Wrong instrument Check that a configuration with
configuration is autopolarizers is loaded.
loaded
Bad cable connec- With the system power off, recheck
tions cable connections.
Computer hang-up Exit the software, and reboot the
system and host computer.
10-20
Automated Polarizers
6
cps on S, <
®
or glycogen concen-
6
cps in VV.
6
cps