Horiba Scientific FluoroMax-4 Operation Manual page 85

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
Dissolved solids
Solid samples, such as crystals, sometimes
are dissolved in a solvent and analyzed in
solution. Solvents, however, may contain
organic impurities that fluoresce and mask
the signal of interest. Therefore, use high-
quality, HPLC-grade solvents. If back-
ground fluorescence persists, recrystallize
the sample to eliminate organic impurities,
and then dissolve it in an appropriate sol-
vent for analysis.
Biological samples
For reproducible results, some samples
may require additional treatment. For ex-
ample, proteins, cell membranes, and cells in solution need constant stirring to prevent
settling. Other samples are temperature-sensitive and must be heated or cooled to en-
sure reproducibility in emission signals.
Remove or change these metal spacers.
5-3
Optimizing Data
Solid-
sample
holder