Using Corrected Signals In Fluoressence - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)

Using corrected signals in FluorEssence™

Introduction
Subtracting blanks, removing dark noise, and correcting for inhomogeneities in the in-
strument or detector response give more accurate spectra. Take special precautions to
incorporate these functions properly into a FluorEssence™ experiment.
Method
Any corrected signal (with a lower-case "c") or algebraic use of corrected signals must
explicitly include all desired corrected signals in the Formulas list. Corrected signals
include:
Dark Offset
Blank Subtraction
Correction-factor file
Note:
set to eliminate generation of a baseline artifact. This artifact is
caused by inappropriate multiplication of dark counts not related to
the spectral response.
An example is provided on the next page.
To use a correction-factor file, you must also activate Dark Off-
4-28
Data Acquisition