Horiba Scientific FluoroMax-4 Operation Manual page 33

® ®
FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
In the FluoroMax
lamp, is available also. A FluorEssence™-controlled mo-
tor and gearbox rotate the mirror in the illumination area,
in order to switch between the CW source and the flash
lamp.
Right: Motor and gearbox that rotate the mirror
to switch lamps (FluoroMax
Monochromators (2 and 4)
Excitation monochromator.
The FluoroMax
sion. The Czerny-Turner design uses all-reflective optics to maintain high resolution
over the entire spectral range, eliminate chromatic aberration for high light-throughput,
and minimize spherical aberrations and re-diffraction.
Gratings
The essential part of a monochromator is a reflection grating. A
grating disperses the incident light by means of its vertical
grooves. A spectrum is obtained by rotating the gratings, and re-
cording the intensity values at each wavelength. The gratings in
the FluoroMax
330 nm (excitation) and 500 nm (emission). Blazing is etching the
grooves at a particular angle, to optimize the grating's reflectivity
in a particular spectral region. The wavelengths selected are opti-
mal for excitation in the UV and visible, and for emission in the
high-UV to near-IR. Each grating is coated with MgF
tion against oxidation. The system uses a direct drive for each grating, to scan the spec-
trum at up to 80 nm s
Slits
The entrance and exit ports of each monochromator have continuously adjustable slits
controlled by FluorEssence™. The width of the slits on the excitation monochromator
determines the bandpass of light incident on the sample and the spectral resolution. The
emission monochromator's slits control the spectral resolution and intensity of the fluo-
rescence signal recorded by the signal detector.
®
-4P, a second source, a xenon flash
®
-4P only).
®
-4 contains Czerny-Turner monochromators for excitation and emis-
®
-4 contain 1200 grooves mm
–1
, with accuracy better than 0.5 nm, and repeatability of 0.3 nm.
Emission monochromator.
–1
, and are blazed at
for protec-
2
2-3
System Description
Xe flash lamp
®
(FluoroMas -4P
only)