13: Glossary - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)

13: Glossary

Absorption
Absorbance
Acquisition modes (R, S
channels)
r
Anisotropy (< >)
Autopolarizers
Bandpass
Bandpass filter
Bioluminescence
Transition, when a photon enters a molecule, from the ground state to
the excited singlet state. This process typically occurs in ~10
The extent of light absorption by a substance. Absorbance, A = –log T,
where T is the transmittance of the sample. Absorbance is also synon-
ymous with optical density, OD. Absorbance can be calculated using
the Beer-Lambert Law:
A = εcl = OD = –log T
ε = extinction coefficient (M
C = sample concentration (M)
l = path length (cm).
The logical input channels used on the spectrofluorometer to input col-
lected signal from the detectors present on the system. The detectors
are assigned as: the reference detector connected to channel R, and the
emission connected to channel S. These logical channel names are
used in the collection of data in most FluorEssence™ applications. The
user may create algebraic expressions on these input channels when
defining experiments in FluorEssence™ (e.g., S/R).
A measurement of the fluorescence polarization of a samples, defined
as the linear-polarizer's component's intensity divided by the total light
intensity. The measurement of anisotropy can provide insight into mo-
lecular size and shape, as well as the environment that surrounds it.
An automated device to hold and precisely rotate a set of polarizers to
acquire anisotropy (or polarization) measurements. FluoroMax
tems with autopolarizers contain two automated polarizer mounts, one
for the excitation polarizer and one for the emission polarizer. Both are
located between the sample compartment and their respective mono-
chromators. Their calibration is maintained by optical sensors that are
offset in the software. Autopolarizers on the FluoroMax
serted into and out of the light path in FluorEssence™, in the Experi-
ment Setup window, under the Accessories icon. (Automated rea-
lignment of the polarizers also may be performed here.)
The wavelength range of light passing through the excitation and emis-
sion spectrometers. The wider the bandpass, the higher the signal in-
tensity.
Optical element that selectively transmits a narrow range of optical
wavelengths.
Emission of light originating from a chemical reaction in a living or-
–1 –1
cm )
13-1
Glossary
–15
s.
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