Horiba Scientific FluoroMax-4 Operation Manual page 219

® ®
FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
Current input module
(DM303)
Cut-on filter
Cut-off filter
Dark counts
Dark offset
Datafile
Dispersion
Emission
monochromator
Emission scan
spectrofluorometer control, timing, and data-acquisition for measure-
ments on the system. The boards carry by default two acquisition
channels, and are linked to all monochromator and accessory control
boards on the underside of the instrument. The CTI card fits into a slot
in the motherboard on the rear of the FluoroMax
The current input module collects the current signal from the reference
photodiode, digitizes the data, and sends it to the CTI card for data-
processing. This module is located directly behind the reference photo-
diode. It has linear response from 0–10 μA.
Optical component that passes light of a higher wavelength.
Optical component that passes light of a lower wavelength.
Inherent background signal measured in counts s
the photomultiplier tube when high voltage is applied. Typically, the
R928P photomultiplier tube used for the FluoroMax
dark counts < 1000 cps.
The software correction used to subtract dark counts (or dark signal) on
a detector from a spectral acquisition. This option appears as a check-
box in the FluorEssence™ software. Use a corrected signal channel for
the acquisition (e.g., S) in order to run the Dark Offset correction.
A file used to store spectral data, constant-wavelength analysis data, or
other recorded data. In FluorEssence™, the most common datafile is
the spectral file (.SPC). This is the file-type that contains spectra ac-
quired from a scan run from the Experiment Setup menu (e.g., emis-
sion scan, time-base scan, single-point, etc.).
The range of wavelengths of light across the field of view of the en-
trance and exit apertures. Dispersion depends on the focal length of the
monochromator, the groove density of the optics, and the f-number
(speed) of the monochromator. Dispersion is usually expressed in na-
nometers of spectral coverage per millimeters of slit width (nm/mm).
The monochromator located after the sample compartment used to iso-
late discrete wavelength components of the sample's fluorescence, and
may be used to scan the emission from a sample. The emission
monochromator on the FluoroMax
monochromator with a Czerny-Turner design: the monochromator in-
cludes a collimating mirror, the reflection grating (blazed at 500 nm),
and a focusing mirror, with slit apertures at the entrance and exit. The
emission-photomultiplier detector is connected to the exit of this
monochromator to measure the fluorescence emission.
Shows the spectral distribution of light emitted by the sample. During
an emission scan, the excitation monochromator remains at a fixed
wavelength while the emission monochromator scans a selected region.
®
–1
®
-4 is an 0.18-m single
13-3
Glossary
-4.
(cps) observed on
®
-4 system has