1939 Cut-On Filter - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012) Components & Accessories
J1939 Cut-On Filter
The model
J1939 Cut-On
Filter set con-
sists of five 2"
× 2" filters with
cut-on wave-
lengths of 370
nm, 399 nm,
450 nm, 500 nm, and 550 nm. To position the filter properly, the FM-2008 Filter Hold-
er is required. The sample compartment has slots to hold the Model FM-2008 filter
holder in the emission and excitation light-path positions. To eliminate second-order ef-
fects from an excitation spectrum, install the filter holder and the appropriate cut-on fil-
ter in the excitation light path.
Cut-on filters eliminate second-order effects of the gratings. For example, if sample ex-
citation is at 300 nm, a second-order peak occurs at 600 nm. If the emission spectrum
extends from 400 nm to 650 nm, a sharp spike occurs at 600 nm. This peak is the se-
cond-order peak of the excitation monochromator. To remove this unwanted peak in
the emission spectrum, place a 350 nm filter in the emission slot. Cut-on filters typical-
ly are used for phosphorescence measurements, where second-order effects are com-
mon.
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