7: Troubleshooting; Chart - Horiba Scientific FluoroMax-4 Operation Manual

® ®
FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)

7: Troubleshooting

The FluoroMax
there is a problem, examine the chart below, and try the steps on the following pages.
Problem
Light is not reaching
the sample.
Signal intensity is
low.
Signal intensity is at
least 10 times lower
than normal.
®
-4 system has been designed to operate reliably and predictably. If
Possible Cause
Excitation shutter closed.
Slits are not open to the
proper width.
®
FluoroMax -4P is in
phosphorimeter layout.
Monochromator is
miscalibrated.
Sample turret is not in
correct position.
CW lamp is not aligned.
Slits are improperly set.
Shutter(s) is(are) not
completely open.
Lamp power-supply is
set to the wrong current
rating.
Lamp is too old.
Shutter(s) closed.
Polarizer is in the light
path.
Monochromators are set
to wrong wavelength.
Detectors are saturated.
Optical density effects
Remedy
Using the software, open the shut-
ter.
Adjust the slits.
Change to a steady-state layout.
Check and recalibrate
monochromator.
Using FluorEssence™, set the
position and open the cover to
verify the position.
Align the lamp.
Adjust the slit widths.
Open the shutter(s) in Real Time
Control.
Call the Service Department.
Replace lamp. (150-W lamp has
lamp lifetime 1200–1500 h.)
Open all shutters in Real Time
Control.
In Real Time Control, move the
polarizer out of the light path.
Select appropriate wavelength
based on excitation and emission
of sample.
Adjust slits. (Signal detector is
linear to 2 × 10
counting mode. Reference detec-
tor saturates at 200 μA.)
Sample is too concentrated. Dilute
7-1
Troubleshooting
6
cps in photon-