Horiba Scientific FluoroMax-4 Operation Manual page 148

® ®
FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
Sample
Window
Time per
flash
Flash
count
For more information about FluorEssence™ phosphorimeter commands, consult the
FluorEssence™ on-line help.
removes any interference from the lamp.
Initial Delay can be varied with time to yield a decay curve. Spectra can
be scanned to isolate different phosphorescing components based on the
lifetime of the luminescent species in the sample. Together, these two
techniques can be used to create three-dimensional plots. For example,
successive scans with varying delay times can be plotted.
To record fluorescence and phosphorescence emission, set Initial Delay to
zero.
Sets the duration of signal acquisition, in ms. The Sample Window opens
when the Initial Delay ends. When the Sample Window opens, the signal
is counted and integrated. After the Sample Window closes, any signal is
ignored.
The Sample Window may be set from 0.01 to 10 000 ms. If the lifetime
of the phosphorescence is known, set the Sample Window to 5–10 times
the lifetime. If the phosphorescence lifetime is unknown, make the Sam-
ple Window a small fraction of the anticipated lifetime, and then increase
it until acceptable results are observed.
If the Sample Window is too long, the detector will record spurious
background signal. If the Sample Window is too short, components of
the lifetime decay may be missed. With two or more species decaying
simultaneously, try varying the Initial Delay and the Sample Window.
Sets the total cycle length per flash, including on time, decay time, and
dead time between flashes. The Time per flash is the reciprocal of the
repetition rate of the lamp pulses. The allowable repetition rate is 0.03–25
Hz. The Time per flash must be slow enough to let the Sample Window
close before another flash begins. Accuracy of the repetition rate is ± 1 ms.
In a Decay by Delay type of scan, the Time per flash is governed by
Time per Flash ≥ Maximum Delay + Sample Window + 20 ms
In a Decay by Window type of scan, the Time per Flash is governed by
Time per Flash ≥ Initial Delay + Maximum Sample Window + 20 ms
Sets the number of lamp pulses that contribute to each data point. The
range for Flash count is 1 to 999. The signal collected per flash is inte-
grated over the total Flash counts before FluorEssence™ stores it. The
more flashes accumulated, the higher the signal-to-noise ratio becomes.
FluoroMax
9-4
®
-4P Phosphorimeter Operation