Calculating Emission Correction Factors - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jun 2012)

Calculating emission correction factors

Introduction
For more information about the theory and application of radiometric correction, con-
sult Accuracy in Spectrophotometry and Luminescence Measurements, Mavrodineau,
Schultz, and Menis, NBS Spec. Publ. 378 (1973), especially p. 137, "Absolute
Spectrofluorometry," by W.H. Melhuish.
Irradiance values for a standard lamp, packaged with the lamp, usually are expressed in
–6 –2
10 W·cm ·nm. With photon-counting systems like the FluoroMax
spectrofluorometer, however, data usually are collected in units of photons·s
To convert the units, multiply each irradiance value by the wavelength at which it is
valid. (The data will still be off by a factor of c, but normalizing the correction factors
compensates for this.) Such a mathematical procedure can be done in a spreadsheet
program.
1
Load the irradiance values.
a
b
Enter the irradiance values into a spreadsheet.
Load the irradiance file supplied by HORIBA Scientific by opening the
FluorEssence™ software, choose File in the toolbar, choose Import, and
then choose SPC file.
Save the file as IRR.
Now you have the two files: IRR and stdlamp2. These files are re-
quired to calculate the emission correction factors for the FluoroMax
8-3
Producing Correction Factors
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-4
–1 –2
·cm ·nm.
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-4.