Horiba Scientific FluoroMax-4 Operation Manual page 147

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
This sequence of excitation, delay, and sampling, is
repeated for each lamp flash. The signal is accumu-
lated for a predetermined number of excitation puls-
es, then FluorEssence™ collects the total signal. Af-
ter collection, FluorEssence™ displays the intensity
of the luminescence as a function of time or wave-
length. The x-axis is based on one of the four scan
options:
Decay by Delay
Decay by Window
Emission
Excitation
Phosphorimeter parameters
Four FluorEssence™ parameters govern the sequence in a phosphorimeter experiment.
These parameters automatically appear on the phosphorimeter experiment-acquisition
window.
Initial De-
lay
Sets the time, in ms, between the start of the lamp flash and the onset of
data-acquisition (opening of the Sample Window). Initial Delay can
range from 0–10 000 ms, in increments of 0.001 ms. Accuracy of Initial
Delay is better than ± 0.001 ms.
Set Initial Delay long enough so that fluorescence emission and lamp de-
cay are complete, so that the resulting spectrum represents phosphores-
cence only. The full-width at half-maximum lamp-pulse width is 3 μs, but
there is a long decay time for the light output. Lamp intensity falls to less
than 1% of peak output after 45 μs. Setting the delay to > 50 μs effectively
FluoroMax
9-3
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-4P Phosphorimeter Operation