Generating Emission Correction Factors - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jun 2012)

Generating emission correction factors

Generate a new correction-factor file only when the gratings or detectors have been re-
placed with those of different specifications than the original hardware.
Required kits
Emission correction factors should be updated periodically or whenever different grat-
ings or signal detectors are installed. The correction factors can be updated either at the
user's location, or by a representative from the Service Department. To arrange for a
visit and a fee estimate, call the Service Department. To update the correction factors
without a service visit, call the Service Department for instructions and further infor-
mation.
One way to generate correction factors for the instrument is to scan the spectrum of a
standard lamp. Because the actual irradiance values of the standard lamp as a function
of wavelength are known, dividing the irradiance values by the lamp spectrum results
in a set of relative correction values. These values can then be applied to the raw fluo-
rescence data. The emission correction factor file mcorrect.spc was acquired in
this manner.
To generate emission correction factors, several items are needed: a standard lamp, ap-
propriate holders, and a light-scattering assembly. HORIBA Scientific offers the F-
3026 Standard Lamp Accessory.
The F-3026 Standard Lamp Assembly is a complete correction-factor kit, which in-
cludes the following items:
Calibrated lamp
Irradiance values for the calibrated lamp
Power supply
Neutral-density filter
Sample-compartment cover
8-2
Producing Correction Factors