Horiba Scientific FluoroMax-4 Operation Manual page 227

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
Synchronous scan
TCSPC
Technical spectrum
Temperature scan
Throughput
Time-based scan
Total luminescence
spectroscopy (TLS)
Transmission
Triplet state (T )
1
energy and the fluorescence peak of maximum energy
Scan type characterizing the overlap between the excitation and emis-
sion. The excitation and emission spectrometers are scanned at the
same time, with a constant offset specified in either nanometers (wave-
–1
length units) or in cm (energy units).
Time-correlated single-photon counting. Technique in which the sam-
ple is excited by a pulsed source, and the sample's fluorescence is col-
lected over the course of many pulses. The arriving photons are timed
after the excitation. Gradually a decay curve is built up and the sam-
ple's fluorescent lifetime is calculated.
A spectrum acquired on research instrumentation with instrumental
bias remaining in the measurement. This spectrum must undergo prop-
er spectral correction in order to match the theoretical spectrum.
HORIBA Scientific spectrofluorometers offer various methods for
such correction, including spectral correction, dark offset, blank sub-
traction, and others.
A FluorEssence™ Kinetics scan-definition that consists of a particular
scan made across a user-defined temperature range. This scan may be
used to monitor a sample's temperature response, or, more specifically,
to perform a melting curve for a sample. Temperature scans require an
automated bath compatible with FluorEssence™ to be attached to the
spectrofluorometer system along with a thermostattable sample mount.
The amount of light that passes through the spectrofluorometer for a
particular measurement. The throughput usually is measured as the
counts per second measured on the water Raman band at 350-nm exci-
tation with 5-nm bandpass. As bandpass increases, so does the
throughput. Like bandpass, throughput has an inverse relationship with
resolution. When the throughput is increased, the resolution decreases.
Scan type in which the sample signal is monitored as a function of
time, while both the excitation and the emission spectrometers remain
at fixed wavelengths. Time-based data are used to monitor enzyme ki-
netics, dual-wavelength measurements, and determine reaction-rate
constants.
Spectroscopy devoted to monitoring changes to the entire excita-
tion/emission matrix of luminescence on a sample. This discipline is
best applied to fast kinetics measurements of samples during reactions,
temperature curves, or changes in other parameters.
Light that passes through a sample without being absorbed, scattered,
or reflected. Transmission is usually measured as a percentage of the
incident light at a certain wavelength.
The spin-paired ground or excited state formed from the excited singlet
13-11
Glossary