Horiba Scientific FluoroMax-4 Operation Manual page 78

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
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Set the scan parameters.
Use the emission maximum determined above for the excitation, use 250
nm for starting, enter the emission maximum minus 15 nm for the end of
the scan, and select two acquisition modes, S and S/R. S collects raw
signal from the emission detector, and S/R ratios the signal to the refer-
ence detector.
Set excitation and emission slits identical to the emission scan.
Be sure that the emission scan did not exceed 2 × 10
signal detector.
Enter the Experiment File name.
Click the Run button
The scan starts.
The resulting spectrum shows maximum excitation wavelength. If the
raw S-channel signal > 2 × 10
Note the excitation peak.
This is the optimum excitation peak position.
.
–1
6
counts s , reduce slits, and rescan.
4-26
Data Acquisition
–1
6
in the S
counts s