8: Producing Correction Factors; Introduction - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jun 2012)

8: Producing Correction Factors

Introduction

Gratings, detectors and other spectrometer components have response characteristics
that are functions of wavelength. These characteristics are superimposed on spectra,
and may yield a potentially misleading trace. For accurate intensity comparisons, such
as those required for quantum-yield determinations, spectrometer-response characteris-
tics must be eliminated. Corrections are made for each of these potential problems by
using radiometric correction factors.
Supplied with the FluoroMax
designed to eliminate response characteristics. These files, xcorrect.spc and
mcorrect.spc, are included with the software and should be copied to the hard
disk. The excitation correction range is from 240–850 nm, and the correction range for
emission spectra is from 290–850 nm.
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-4 are sets of excitation and emission correction factors
8-1
Producing Correction Factors