Horiba Scientific FluoroMax-4 Operation Manual page 46

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
Emission-monochromator calibration check
Note:
rectly affected by the calibration of the excitation monochromator.
This calibration check verifies the wavelength calibration of the emission
monochromator with the emission photomultiplier tube. It is an emission scan of the
Raman-scatter band of water performed in right-angle mode. Perform this check after
the xenon-lamp scan. When completed, the performance of the system has been veri-
fied.
The water sample should be research-quality, triple-distilled or de-ionized water.
HPLC-grade (18-MΩ spec.) or equivalent water is suggested for the Raman scan. Im-
pure samples of water will cause elevated background levels as well as distorted spectra
with (perhaps) some unwelcome peaks.
Use a 4-mL quartz cuvette.
Note:
cence or filtering effects.
1
Insert the water sample into the sample com-
partment.
With an automated sample changer, note the position number in which the sam-
ple cell is placed.
2
Close the lid of the sample chamber.
3
In the main FluorEssence toolbar, choose the
Experiment Menu button
The Fluorescence Main Experiment
Menu opens.
4
Choose Spectra.
The Experiment Type window appears:
The emission-monochromator calibration of the instrument is di-
Avoid glass or acrylic cuvettes: they may exhibit UV fluores-
:
3-10
System Operation