Horiba Scientific FluoroMax-4 Operation Manual page 76

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
f
g
h
Click the Experiment File field, and enter a new file name or select a
previously saved file.
Verify that experimental parameters are correct.
Be sure to check all parameters under all icons in the left-hand column.
Set the scan parameters.
Most of these parameters are a trade-off between speed and precision.
Choose integration time, increments, and number of scans judiciously, to
give an accurate result without excessive time spent. HORIBA Scientific
suggests an increment of 1.0 nm, an integration time of 0.1–0.5 s, and
one scan. If unsure of an excitation wavelength, try 300 nm, at which
many samples absorb light. Use S (signal detector) for the acquisition
mode. Don't forget a data file name.
Note:
To minimize Rayleigh scatter, offset the start posi-
tion by at least 15 nm from the excitation wavelength,
with a bandpass of 5 nm. For example, for an excitation
wavelength of 300 nm, use 315 nm as the start. Set the
ending wavelength to 550 nm. Use an increment of 2 nm
and an integration time of 0.1 s.
4-24
Data Acquisition