4: Data Acquisition; Introduction To Fluoressence™ And Multigroup - Horiba Scientific FluoroMax-4 Operation Manual

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)

4: Data Acquisition

Introduction to FluorEssence™ and
Multigroup
This chapter presents an introduction to some of the special buttons used in
FluorEssence™ to record and present data with the FluoroMax
cated in FluorEssence ™'s main window, are:
Auto Run Previous Ex-
periment
Experiment
Menu
Previous Ex-
periment
Run JY Batch
Experiments
For a detailed description of these FluorEssence™ routines, see the FluorEssence™
User's Guide and on-line help.
A second program, called Multigroup, offers repeated and sequential fluorescence ex-
periments. Functions such as delays, temperature ramps, ratiometric probes such as
Fura-2, and multiple samples and wavelength-groups are allowed within Multigroup.
You can start Multigroup from within FluorEssence™, or from a Windows
icon.
In addition, methods for determining best excitation and emission wavelengths are pre-
sented, in case these wavelengths are unknown for the sample.
3D Scan to 3D
Profile
Real Hide Launch
Time Events DataStation
Control
Make Multigroup
Overlay
File
Show Launch
Events DataStation
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-4. These buttons, lo-
Rescale Quantum Yield cal-
Y culator
Switch menu between
HJY Software Applica-
tion and Origin Std.
Create/Use Calibration 2D Intensi-
Curve from CWA Data ty Map
Convert XYY Overlay
data to Con- graph(s)
tour Plot
4-1
Data Acquisition
Blank Subtrac-
tion
View Experi-
ment Settings
Normalize
Data
Extract Experiment
file from Data
(Notes)
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desktop