Horiba Scientific FluoroMax-4 Operation Manual page 150

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
Isolate components in a mixture based on lifetimes
To the right are three scans
of an aqueous mixture of
terbium and europium chlo-
rides that isolate different
phosphorescent components
based on their lifetimes. For
example, a sample contain-
ing two phosphorescent
species with different life-
times (Tb = 421 μs; Eu =
113 μs) can be resolved into
its components by varying
the Initial Delay parameter,
here shown as bold italic
numerals, 0.1 ms, 1 ms,
and 2 ms initial delay. No-
tice the wavelength shift in
the 593 nm peak (marked
with a gray line) from ini-
tially mostly europium fluorescence (short lifetime) to a mixture of terbium (longer
lifetime) and europium fluorescence at later delays. Also notice the Eu peak near 690
nm at 0.1 ms that vanishes at later times. This experiment was performed using the
Emission subtype of Phos experiment.
Three-dimensional plots
Using the phosphorescence delay curve with the Initial Delay technique, a three-
dimensional graph can be created, as shown below. This graph shows successive scans
of a mixture of Tb-L and Eu-L (where L = benzophenone antenna chromophore) with
varying time delays, segregating species into contours representing a particular slice of
time.
FluoroMax
9-6
®
-4P Phosphorimeter Operation