Horiba Scientific FluoroMax-4 Operation Manual page 79

® ®
FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
3
Find the optimal emission peak.
a
b
Use the optimum excitation wavelength determined in step 2.
When complete, both excitation and emission peaks are found.
Optimized excitation and emission spectra of a 1 × 10
solution are shown below. Because the acquisition modes were different
for the excitation and emission scans, the data intensity had to be nor-
malized. After normalization, the excitation and emission scans are vir-
tually mirror images of one another.
Normalized excitation and emission spectra of a 1 × 10
anthracene solution.
4-27
Data Acquisition
–8
-M anthracene
M
-
–8