Horiba Scientific FluoroMax-4 Operation Manual page 220

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FluoroMax -4 & FluoroMax -4P with USB rev. D (30 Jul 2012)
Energy transfer
Excitation/emission ma-
trix (EEM)
Excitation
monochromator
Excitation scan
Excited state (S )
1
Experiment file
Extrinsic fluorescence
Filter
The transfer of the excited energy from a donor to an acceptor. The
transfer occurs without the appearance of a photon and is primarily a
result of dipole-dipole interactions between the donor and acceptor.
A three-dimensional plot showing the total luminescence from a sam-
ple across all useful wavelengths. Total luminescence spectroscopy is
devoted to measurements of these EEMs for various materials. See al-
so: Total Luminescence Spectroscopy
The monochromator, located between the xenon lamp and the sample
compartment, used to isolate discrete wavelength components of the
excitation beam. This beam is directed to the sample, during which the
excitation monochromator may be used to scan the excitation spectrum
from a sample. The excitation monochromator on the FluoroMax
an 0.18-m single monochromator with Czerny-Turner design. This
means that the monochromator includes a collimating mirror, the re-
flection grating (blazed at 330 nm), and a focusing mirror, with slit ap-
ertures at the entrance and exit. An excitation shutter is located directly
after the excitation exit slit to protect the sample from photobleaching.
The reference detector looks at a fraction of the light exiting the excita-
tion monochromator to correct for the lamp response, if desired.
Shows the spectral distribution of light absorbed by the sample. To ac-
quire an excitation scan, the excitation monochromator scans a selected
spectral region while the emission monochromator remains at a fixed
wavelength.
The energy level to which an electron in the ground level of a molecule
is raised after the absorption of a photon of a particular wavelength.
Subsequently, fluorescence occurs, if the molecule returns to the
ground state via a radiative transfer from the S
state.
A file that contains specific information on the experimental setup for
an acquisition defined in Experiment Setup. This file is saved with a
default *.EXP extension. In addition to basic scan parameters, this file
saves system defaults (such as slit units), and some accessory settings
for the acquisition. Each acquisition type in the Fluoresence Experi-
ment Menu has its own default experiment file (e.g., DfltEm1.xml
is the default emission-scan definition). Use experiment files to archive
scan settings for acquisitions that are performed routinely.
Inherent fluorescence of probes used to study non-fluorescent mole-
cules.
An optical element that is used to select certain wavelengths of light.
Types of filters include high-pass, low-pass, bandpass, and neutral den-
sity.
state to the ground
1
13-4
Glossary
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-4 is