Intensity Test - Agilent Technologies 1220 Infinity II LC User Manual

9
Test Functions and Calibration
Diode Array Detector (DAD)
Test Failed
Probable cause
1
2
3

Intensity Test

The test is for the standard flow cells (10 mm and 6 mm pathlength) only. The nano-flow
N O T E
cells (80 nL and 500 nL) cannot be run with this test due to its low volume.
The intensity test measures the intensity of the deuterium and tungsten lamps
over the full wavelength range (190 – 950 nm). Four spectral ranges are used
to evaluate the intensity spectrum. The test is used to determine the
performance of the lamps and optics (see also
the test is started, the 1-nm slit is moved into the light path automatically, and
the gain is set to zero. To eliminate effects due to absorbing solvents, the test
should be done with water in the flow cell. The shape of the intensity
spectrum is primarily dependent on the lamp, grating, and diode array
characteristics. Therefore, intensity spectra will differ slightly between
instruments.
Intensity Test Evaluation
The Agilent LabAdvisor, ChemStation and Instant Pilot evaluate four spectral
ranges automatically, and display the limits for each range, the measured
intensity counts, and passed or failed for each spectral range (see
on page 178).
176
Defective slit assembly (stray light).
Defective detector main board.
Defective PDA/optical unit.
Figure 54
Suggested actions
Run the
"Self-test"
Please contact your Agilent service
representative.
Please contact your Agilent service
representative.
on page 178 shows a typical intensity test spectrum.
"Slit Test" on page 174 (part of the
on page 171).
"Cell Test" on page 184). When
1220 Infinity II LC System User Manual
Figure 54