Agilent Technologies 1220 Infinity II LC User Manual page 146

9
Test Functions and Calibration
Solvent Delivery System
Plateau 2
The flow is reduced to 2 μL/min for approximately one minute (plunger 1 still
delivering). The pressure during the plateau should remain constant or
increase slightly. A falling pressure indicates a leak of >2 μL/min.
Ramp 3
The flow increases to 220 μL/min and the stroke is changed to 100 μL. Plunger
1 completes its stroke. Next, the flow is changed to 510 μL/min. The ramp
reaches 390 bar with the plunger sequence 2-1-2-1.
Plateau 3
When the system pressure reaches 390 bar, the flow is reduced to zero, and
the pressure stabilizes just below 400 bar.
One minute after reaching the maximum pressure, the pressure drop should
not exceed 2 bar/min.
146 1220 Infinity II LC System User Manual