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Agilent Technologies G7166A User Manual

Preparative valve-based fraction collector
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Agilent 1260 Infinity II
Preparative Valve-Based
Fraction Collector
User Manual
Agilent Technologies

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Summary of Contents for Agilent Technologies G7166A

  • Page 1 Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector User Manual Agilent Technologies...
  • Page 2 Notices Warranty © Agilent Technologies, Inc. 2016-2018 receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June No part of this manual may be reproduced The material contained in this docu- 1987). U.S. Government users will receive in any form or by any means (including ment is provided “as is,”...
  • Page 3 In This Guide In This Guide This manual contains technical reference information about the Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector. 1 Introduction to the Valve-Based Fraction Collector This chapter gives an introduction to the module, instrument overview and internal connectors.
  • Page 4 In This Guide 8 Maintenance This chapter gives you an overview and instructions about the possible maintenance and repair procedures that can be performed by the user. 9 Parts and Materials This chapter provides information on parts and materials. 10 Identifying Cables This chapter provides information on cables used with the module.

  • Page 5: Table Of Contents

    Contents Contents 1 Introduction to the Valve-Based Fraction Collector Product Description Features Typical Applications Mounting Examples 2 Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications 3 Installing the Preparative Valve-based Fraction Collector Unpacking the module Installing the Preparative Valve-Based Fraction Collector Flow connections with the Preparative Valve-Based Fraction Collector 4 Using the Preparative Valve-Based Fraction Collector Configuration and Operation of the Fraction Collector...
  • Page 6 Contents 6 Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software Hardware Symptoms 7 Error Information What Are Error Messages General Error Messages Module Specific Error Messages 8 Maintenance Introduction to Maintenance Cautions and Warnings Overview of Maintenance Cleaning the Module Replacing the Fuses of the Preparative Valve-Based Fraction Collector Replacing the Module Firmware 9 Parts and Materials...
  • Page 7 Contents 11 Hardware Information Electrical Connections Interfaces Configuration Switch Settings of the Preparative Valve-Based Fraction Collector 12 Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Agilent Technologies on Internet Preparative Valve-Based Fraction Collector...
  • Page 8 Contents Preparative Valve-Based Fraction Collector...

  • Page 9: Introduction To The Valve-Based Fraction Collector

    Preparative Valve-Based Fraction Collector Introduction to the Valve-Based Fraction Collector Product Description Features Typical Applications Mounting Examples This chapter gives an introduction to the module, instrument overview and internal connectors. Agilent Technologies...

  • Page 10: Product Description

    Product Description The Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector (G7166A) comprises 11 collection valves, one waste valve and one solvent inlet port. Solvent coming from a preparative HPLC system can be diverted into 11 different fractions depending on the software/firmware settings. The valves can then be blown clean by allowing compressed air or nitrogen to be passed through the same valves.

  • Page 11: Features

    Introduction to the Valve-Based Fraction Collector Features Features The 1260 Infinity II Preparative Valve-Based Fraction Collector (G7166A) includes the following features: • Built-in power supply • Leak handling with a leak pane and a leak sensor underneath the valve head •...

  • Page 12: Typical Applications

    Introduction to the Valve-Based Fraction Collector Typical Applications Typical Applications The Preparative Valve-based Fraction Collector allows the user to collect large fractions from a preparative HPLC run, at high flow rates, while minimizing carry-over, due to compressed air (or nitrogen) blowing clean the fraction lines.

  • Page 13: Mounting Examples

    Introduction to the Valve-Based Fraction Collector Mounting Examples Mounting Examples Figure 2 Mounting Examples for the Preparative Valve-based Fraction Collector Preparative Valve-Based Fraction Collector...
  • Page 14 Introduction to the Valve-Based Fraction Collector Mounting Examples Preparative Valve-Based Fraction Collector...

  • Page 15: Site Requirements And Specifications

    Preparative Valve-Based Fraction Collector Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 16: Site Requirements

    Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 19.
  • Page 17 Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Power cords WA R N I N G Solvents may damage electrical cables.
  • Page 18 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 1 on page 19) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.

  • Page 19: Physical Specifications

    Site Requirements and Specifications Physical Specifications Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 1.9 kg (4.2 lbs) Dimensions 95 x 95 × 300 mm (height × width × depth) (3.7 x 3.7 × 11.8 inches) Line voltage 100 –...

  • Page 20: Performance Specifications

    Site Requirements and Specifications Performance Specifications Performance Specifications Table 2 Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector (G7166A) Performance Specifications Type Specification Number of solvent channels 13 (including inlet and waste) Fittings ¼-28 External leak sensor YES, same function as internal sensor Solvent Flow range 0 –...
  • Page 21 Mount the Preparative Valve-Based Fraction Collector to an Instrument by Using a Valve Rail Mount the Preparative Valve-Based Fraction Collector to a G1383A Column Organizer Flow connections with the Preparative Valve-Based Fraction Collector This chapter gives information about the installation of the module. Agilent Technologies...

  • Page 22: Installing The Preparative Valve-Based Fraction Collector Unpacking The Module

    Unpacking the module Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C AU T I O N If there are signs of damage, please do not attempt to install the module.
  • Page 23 Ensure all parts and materials have been delivered with your module. The delivery checklist is shown below. For parts identification please check the illustrated parts breakdown in Parts and Materials. Please report any missing or damaged parts to your local Agilent Technologies sales and service office. Table 3 Delivery Checklist...

  • Page 24: Installing The Preparative Valve-Based Fraction Collector

    Installing the Preparative Valve-based Fraction Collector Installing the Preparative Valve-Based Fraction Collector Installing the Preparative Valve-Based Fraction Collector The Preparative Valve-Based Fraction Collector can be installed in different ways. It can be attached to either side of your Instrument with the use of the Valve Rail (InfinityLab LC Series, 1260, 1290 Series Pumps and Detectors, for older modules order new cover kits), or it can be mounted to a G1383A column organizer by using the optional column stand mount.
  • Page 25 Installing the Preparative Valve-based Fraction Collector Installing the Preparative Valve-Based Fraction Collector Slide the first clamp on to one side of the Preparative Slide the leak tray onto the bottom of the Preparative Valve-Based Fraction Collector housing. Valve-Based Fraction Collector. Slide the second clamp on to the same side as the first Connect the leak sensor cable to the Preparative one.

  • Page 26: Mount The Preparative Valve-Based Fraction Collector To An Instrument By Using A Valve Rail

    Installing the Preparative Valve-based Fraction Collector Installing the Preparative Valve-Based Fraction Collector Mount the Preparative Valve-Based Fraction Collector to an Instrument by Using a Valve Rail Figure 3 Possible mounting points to an Agilent InfinityLab LC Series module Parts required Description 5067-5685 Clamp Guide Kit-IF-II...
  • Page 27 Installing the Preparative Valve-based Fraction Collector Installing the Preparative Valve-Based Fraction Collector Ensure the power switch at the rear of the module is OFF Identify the marks for the screws on the side panel of and the power connector is unplugged. the cabinet assy and screw the valve rail to the side panel of the cabinet.
  • Page 28 Installing the Preparative Valve-based Fraction Collector Installing the Preparative Valve-Based Fraction Collector Connect the power cable to the power connector at the Connect compressed air or nitrogen line. rear of the module. Power on the module by switching the Power button at the rear of the module.

  • Page 29: Mount The Preparative Valve-Based Fraction Collector To A G1383A Column Organizer

    Installing the Preparative Valve-based Fraction Collector Installing the Preparative Valve-Based Fraction Collector Mount the Preparative Valve-Based Fraction Collector to a G1383A Column Organizer For more information about the column organizer read the G1383-90011 Column Organizer N O T E Quick Reference Guide. Ensure the power switch at the rear of the module is OFF Assemble the column stand with a system.
  • Page 30 Installing the Preparative Valve-based Fraction Collector Installing the Preparative Valve-Based Fraction Collector Slide the Preparative Valve-Based Fraction Collector Slide the column stand mount onto the column stand. onto the column stand mount. Connect the CAN interface connection. Connect the power cable to the power connector at the rear of the module.

  • Page 31: Flow Connections With The Preparative Valve-Based Fraction Collector

    Installing the Preparative Valve-based Fraction Collector Flow connections with the Preparative Valve-Based Fraction Collector Flow connections with the Preparative Valve-Based Fraction Collector Pollution of sensitive parts C AU T I O N Inaccurate selection of valve ports and bias of collection results. ➔...
  • Page 32 Installing the Preparative Valve-based Fraction Collector Flow connections with the Preparative Valve-Based Fraction Collector 2 For each of the tubes for the valves, place the loosely assembled fittings into the valve ports and make sure the tubing is bottomed out inside the port. Ensure the appropriate fittings are used and insert the tubing fully into the correct N O T E components before tightening the fittings.

  • Page 33: Using The Preparative Valve-Based Fraction Collector

    Preparative Valve-Based Fraction Collector Using the Preparative Valve-Based Fraction Collector Configuration and Operation of the Fraction Collector Delay Volumes and Delay Calibration Solvent Information Material Information Status Indicators This chapter explains the essential operational parameters of the module. Agilent Technologies...

  • Page 34: Configuration And Operation Of The Fraction Collector

    Using the Preparative Valve-Based Fraction Collector Configuration and Operation of the Fraction Collector Configuration and Operation of the Fraction Collector Delay Volumes and Delay Calibration Once software is installed and the Preparative Valve-Based Fraction Collector is ready to be operated, the fraction delay time needs to be determined. Figure 4 on page 34 shows a schematic drawing of the flow path between the detector and the Preparative Valve-Based Fraction Collector with the delay...
  • Page 35 Using the Preparative Valve-Based Fraction Collector Configuration and Operation of the Fraction Collector Performing a Delay Calibration with an UV Detector 1 Place a vial containing the Delay Sensor Calibrant (5190-8223) in position 1 of the Autosampler. 2 Remove the installed column and replace for the delay coil or union. 3 Connect a bottle of water to Channel A 4 Open a session of LAB Advisor and connect to the system with the 1260 Infinity II Preparative Valve-Based Fraction Collector.

  • Page 36: Solvent Information

    Using the Preparative Valve-Based Fraction Collector Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Follow recommendations for avoiding the growth of algae, see pump manuals. • Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 μm filters.

  • Page 37: Material Information

    Using the Preparative Valve-Based Fraction Collector Solvent Information • Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid).
  • Page 38 Using the Preparative Valve-Based Fraction Collector Solvent Information It is stable in the specified pH range (for the Bio-inert LC system: pH 1 – 13, see bio-inert module manuals for details), and inert to many common solvents. There is still a number of known incompatibilities with chemicals such as chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid >...
  • Page 39 Using the Preparative Valve-Based Fraction Collector Solvent Information Tantalum (Ta) Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide. It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides.
  • Page 40 Using the Preparative Valve-Based Fraction Collector Solvent Information This is due to a thin oxide layer on the surface, which is stabilized by oxidizing compounds. Non-oxidizing acids (for example, hydrochloric, sulfuric and phosphoric acid) can cause slight corrosion, which increases with acid concentration and temperature.
  • Page 41 Using the Preparative Valve-Based Fraction Collector Solvent Information Platinum/Iridium Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Fluorinated polymers (PTFE, PFA, FEP, FFKM) Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy) and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents.

  • Page 42: Status Indicators

    Using the Preparative Valve-Based Fraction Collector Status Indicators Status Indicators 1 The module status indicator indicates one of six possible module conditions: Status indicators 1. Idle 2. Run mode 3. Not-ready. Waiting for a specific pre-run condition to be reached or completed.

  • Page 43: Preparing The Preparative Valve-Based Fraction Collector

    Setting up the Preparative Valve-Based Fraction Collector with the Instrument Control Interface Overview Instrument Configuration Additional Instrument Configuration Settings Preparative Valve-Based Fraction Collector User Interface (Dashboard Panel) Method Parameter Settings Advanced Settings Timetable Settings Fraction Preview Pooling This chapter explains the operational parameters of the module. Agilent Technologies...

  • Page 44: Leak And Waste Concept

    Preparing the Preparative Valve-Based Fraction Collector Leak and Waste Concept Leak and Waste Concept The leak concept of the Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector is designed to deal with leaks of solvents specified up to a maximum flow rate of 200 mL/min. In addition to this manual it is important to follow the instructions for the installation of the Leak and Waste Handling procedure in the System Installation Guide, to ensure a reliable leak transfer to the waste bottle.

  • Page 45: Best Practices

    Preparing the Preparative Valve-Based Fraction Collector Best Practices Best Practices Weekly Tasks • Flush all channels with water to remove salt deposits, using an intermediate solvent when needed. • Wipe the outside clean with a moist rag. How to deal with solvents •...

  • Page 46: Setting Up The Preparative Valve-Based Fraction Collector With The Instrument Control Interface

    Preparing the Preparative Valve-Based Fraction Collector Setting up the Preparative Valve-Based Fraction Collector with the Instrument Control Interface Setting up the Preparative Valve-Based Fraction Collector with the Instrument Control Interface Overview Parameters described in following sections are offered by the instrument control interface and can usually be accessed through Agilent instrument control software.
  • Page 47 Preparing the Preparative Valve-Based Fraction Collector Setting up the Preparative Valve-Based Fraction Collector with the Instrument Control Interface Table 4 Instrument configuration parameters Parameter Description Communication: The parameters in this dialog box are detected automatically during autoconfiguration. • Device name, •...
  • Page 48 Preparing the Preparative Valve-Based Fraction Collector Setting up the Preparative Valve-Based Fraction Collector with the Instrument Control Interface Table 4 Instrument configuration parameters Parameter Description Peak Detectors • Module type: product number of the peak detector detected during autoconfiguration • Serial number: serial number of the peak detector detected during autoconfiguration •...

  • Page 49: Additional Instrument Configuration Settings

    Preparing the Preparative Valve-Based Fraction Collector Setting up the Preparative Valve-Based Fraction Collector with the Instrument Control Interface Additional Instrument Configuration Settings Table 5 Additional Instrument Configuration Settings Reset Fraction Collector Re-initializes the fraction collector. Modify – Detector Delay Volumes… Opens a window with a table that lists all potential analog peak detection sources configured in your instrument.

  • Page 50: Preparative Valve-Based Fraction Collector User Interface (Dashboard Panel)

    Preparing the Preparative Valve-Based Fraction Collector Setting up the Preparative Valve-Based Fraction Collector with the Instrument Control Interface Preparative Valve-Based Fraction Collector User Interface (Dashboard Panel) Table 6 Preparative Valve-Based Fraction Collector User Interface Parameter Description Module graphic The items in the Preparative Valve-Based Fraction Collector graphic have the following meaning and function: •...
  • Page 51 Preparing the Preparative Valve-Based Fraction Collector Setting up the Preparative Valve-Based Fraction Collector with the Instrument Control Interface Table 6 Preparative Valve-Based Fraction Collector User Interface Parameter Description Context Menu The context menu of the dashboard panel contains the following commands: •...

  • Page 52: Method Parameter Settings

    Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Method Parameter Settings The Preparative Valve-Based Fraction Collector method setup parameters are in eight sections: • Collection Behavior • Peak Triggers • Trigger Combinations • Stoptime • Posttime • Advanced • Timetable •...
  • Page 53 Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Table 7 Method Parameter Settings Parameter Description Collection Behavior Use this setting to either enable or disable the fraction collection parameters of the instrument. Peak Triggers Use the Peak Triggers table to specify the detection settings of the peak detectors available in your system.
  • Page 54 Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Table 7 Method Parameter Settings Parameter Description Trigger Combinations Use the Trigger Combinations to specify how multiple peak triggers are combined to start or stop Fraction Collection. You can choose that: •...

  • Page 55: Advanced Settings

    Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Table 7 Method Parameter Settings Parameter Description Timetable “Timetable Settings” on page 57 Fraction Preview Use the Fraction Preview screen to test the fraction collection parameters against one or more reference signals. You can also use the Fraction Preview to optimize the fraction collection parameters interactively.
  • Page 56 Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Table 8 Advanced Parameters Description Parameter Description Delay Settings Use the Delay Settings table to specify the delay that is applied to a peak trigger signal. You can specify this setting for each peak detector separately.

  • Page 57: Timetable Settings

    Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Timetable Settings A timetable entry is crucial to enable any fraction collection. N O T E Figure 7 Timetable settings Use the Timetable to program changes in the fraction collector parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable.
  • Page 58 Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Table 9 Timetable Functions Function Parameters Fraction Mode • Off (Turns off the current fraction collection, where you use Off to turn off fraction collection at the end of the run if you have not specified a Stoptime) •...
  • Page 59 Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Table 9 Timetable Functions Function Parameters Trigger Settings • Trigger Source (Click the down-arrow and select the trigger source from the drop-down list) • Peak Detection Mode (Click the down-arrow and select the peak detection mode from the drop-down list).

  • Page 60: Fraction Preview

    Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings Fraction Preview To determine the appropriate fraction collection parameters the Agilent ChemStation provides a valuable tool that becomes accessible by pushing the button labelled Fraction Preview Tool (Figure 8 on page 60) in the Peak Detectors section.
  • Page 61 Preparing the Preparative Valve-Based Fraction Collector Method Parameter Settings the right hand side of the Fraction Preview screen the chromatogram can be zoomed, the values for up and down slope can be specified and the upper and lower threshold level can be set-up. The graphically specified values are automatically transferred to the Peak Detector table.

  • Page 62: Pooling

    Preparing the Preparative Valve-Based Fraction Collector Pooling Pooling Pooling is the collection of multiple fractions into the same collection vessel. You can pool fractions from either multiple injections of the same sample or single injections of different samples. Fractions are pooled automatically when you specify multiple injections from the same location in one line of the sequence table, or if the same fraction start location is specified for sequential locations in the sequence table.

  • Page 63: Troubleshooting And Diagnostics

    Agilent Lab Advisor Software Hardware Symptoms Synchronization Lost Leak Calibration delay volume test shows two peaks Valve switch failed Limitations and how to avoid problems This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces. Agilent Technologies...

  • Page 64: User Interfaces

    Troubleshooting and Diagnostics User Interfaces User Interfaces • Depending on the user interface, the available tests and the screens/reports may vary. • Preferred tool should be Agilent Lab Advisor Software, see “Agilent Lab Advisor Software” on page 65. • The Agilent OpenLab ChemStation C.01.03 and above do not include any maintenance/test functions.

  • Page 65: Agilent Lab Advisor Software

    Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent Lab Advisor Software The Agilent Lab Advisor Software is a standalone product that can be used with or without a chromatographic data system. Agilent Lab Advisor helps to manage the lab for high-quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status, Early Maintenance Feedback counters (EMF), instrument configuration information, and diagnostic tests.

  • Page 66: Hardware Symptoms

    Troubleshooting and Diagnostics Hardware Symptoms Hardware Symptoms Synchronization Lost During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.

  • Page 67: Calibration Delay Volume Test Shows Two Peaks

    Troubleshooting and Diagnostics Hardware Symptoms Calibration delay volume test shows two peaks Two peaks have been detected during the delay calibration. Probable cause Suggested actions Check method and delay calibration procedure. Wrong sample has been used for the delay calibration Check flow path for leaks and air bubbles.

  • Page 68: Limitations And How To Avoid Problems

    Troubleshooting and Diagnostics Hardware Symptoms Limitations and how to avoid problems Table 10 Limitations Limitation How to avoid problems Replacing fraction When replacing filled tubes or bottles, make sure to re-set the bottle volumes, otherwise the containers Preparative Valve-Based Fraction Collector will not recognize that the fraction containers were emptied.

  • Page 69: Error Information

    Module Specific Error Messages Initialization of Valve Failed Pressure Cluster Partner Missing Position Cluster Partner Missing This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent Technologies...

  • Page 70: What Are Error Messages

    Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).

  • Page 71: General Error Messages

    Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout Error ID: 0062 The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and The analysis was completed successfully, source of a not-ready condition.

  • Page 72: Remote Timeout

    Error Information General Error Messages Remote Timeout Error ID: 0070 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.

  • Page 73: Leak Sensor Short

    Error Information General Error Messages Leak Sensor Short Error ID: 0082 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.

  • Page 74: Compensation Sensor Open

    Error Information General Error Messages Compensation Sensor Open Error ID: 0081 The ambient-compensation sensor (NTC) on the main board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the main board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes.

  • Page 75: Leak

    Error Information General Error Messages Leak Error ID: 0064 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent.

  • Page 76: Module Specific Error Messages

    Error Information Module Specific Error Messages Module Specific Error Messages Initialization of Valve Failed Error ID: 24000 During the initialization process the motor of the valve drive moves to some special positions depending on the installed valve head. A failure in this process means either that the movement couldn’t be performed properly or it was not noticed correctly by the sensor.

  • Page 77: Position Cluster Partner Missing

    Error Information Module Specific Error Messages Position Cluster Partner Missing Probable cause Suggested actions Check the CAN cable connections of the Communication issues modules. Check and correct if necessary the valve Configuration mismatch configuration and presence of defined position cluster partner. Preparative Valve-Based Fraction Collector...
  • Page 78 Error Information Module Specific Error Messages Preparative Valve-Based Fraction Collector...

  • Page 79: Maintenance

    Cleaning the Module Replacing the Fuses of the Preparative Valve-Based Fraction Collector Replacing the Module Firmware This chapter gives you an overview and instructions about the possible maintenance and repair procedures that can be performed by the user. Agilent Technologies...

  • Page 80: Introduction To Maintenance

    Maintenance Introduction to Maintenance Introduction to Maintenance The module is designed for little to no maintenance. The most frequent maintenances such as cleaning the valve can be done from the front with module in place in the system stack. There are no serviceable parts inside. N O T E Do not open the module.

  • Page 81: Cautions And Warnings

    Maintenance Cautions and Warnings Cautions and Warnings Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
  • Page 82 Maintenance Cautions and Warnings Safety standards for external equipment C AU T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.

  • Page 83: Overview Of Maintenance

    Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance procedures (simple repairs) that can be done without opening the main cover. Table 11 Maintenance Procedures Procedure Typical Frequency “Cleaning the Module” on page 84 If required “Replacing the Fuses of the Preparative When a fuse is defect Valve-Based Fraction Collector”...

  • Page 84: Cleaning The Module

    Maintenance Cleaning the Module Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Port can be cleaned by pumping a suitable solvent through each of the ports, and blowing the ports dry with nitrogen or air.

  • Page 85: Replacing The Fuses Of The Preparative Valve-Based Fraction Collector

    Maintenance Replacing the Fuses of the Preparative Valve-Based Fraction Collector Replacing the Fuses of the Preparative Valve-Based Fraction Collector When If the valve drive shows no reaction. Tools required Description Screwdriver Parts required Description 2110-1486 Fuse 2 AT250 V Electrical shock WA R N I N G ➔...
  • Page 86 Maintenance Replacing the Fuses of the Preparative Valve-Based Fraction Collector To access the fuse drawer, gently lift the outer plastic Pull out the fuse drawer as shown. housing of the power inlet socket using a flat screwdriver. Replace the defect fuse(s). Slide in the fuse drawer and push till it fits tightly.

  • Page 87: Replacing The Module Firmware

    Maintenance Replacing the Module Firmware Replacing the Module Firmware Table 12 Module Specific Information Initial firmware (main and resident) C.07.01 Compatible with 1200 series modules All other modules must have firmware revision A.07.01 or B.07.01 or above (main and resident). Otherwise the communication will not work.
  • Page 88 Maintenance Replacing the Module Firmware Preparative Valve-Based Fraction Collector...

  • Page 89: Parts And Materials

    Preparative Valve-Based Fraction Collector Parts and Materials Valve Drive Parts Accessory Kit This chapter provides information on parts and materials. Agilent Technologies...

  • Page 90: Valve Drive Parts

    Parts and Materials Valve Drive Parts Valve Drive Parts Item Description 5043-0275 Clamp guide For attaching the valve to a rail assembly 5067-4792 Leak sensor assembly External leak sensor 5043-0271 Holder leak plane 5043-0270 Leak plane 2110-1486 Fuse 2 AT250 V 5067-4634 Valve rail assembly “Installing the Preparative Valve-Based Fraction Collector”...

  • Page 91: Accessory Kit

    Parts and Materials Accessory Kit Accessory Kit Accessory Kit (G9352-68100) Item Description 5043-0270 Leak plane 5043-0271 Holder leak plane 5067-4792 Leak sensor assembly 5181-1519 CAN cable, Agilent module to module, 1 m 5043-0275 Clamp guide 2110-1486 Fuse 2 AT250 V 5062-2483 Tube PTFE 1.5 mm x 5 m, 3 mm od 0100-2298...
  • Page 92 Parts and Materials Accessory Kit Preparative Valve-Based Fraction Collector...

  • Page 93: Identifying Cables

    Preparative Valve-Based Fraction Collector Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the module. Agilent Technologies...

  • Page 94: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
  • Page 95 Identifying Cables Cable Overview LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) RS-232 cables (not for FUSION Description board) G1530-60600 RS-232 cable, 2 m RS232-61601 RS-232 cable, 2.5 m...

  • Page 96: Analog Cables

    Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
  • Page 97 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog +...

  • Page 98: Remote Cables

    Identifying Cables Remote Cables Remote Cables ERI (Enhanced Remote Interface) 5188-8029 ERI to general purpose p/n 5188-8029 Color code Enhanced Classic Active (TTL) Remote Remote white START REQUEST brown STOP green READY High yellow POWER ON High grey NOT USED pink SHUT DOWN blue...
  • Page 99 Identifying Cables Remote Cables 5188-8044 ERI to ERI (Connector D_Subminiature 15 pin) Table 13 5188-8044 ERI to ERI p/n 5188-8044 Pin (ERI) Signal Pin (ERI) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector...
  • Page 100 Identifying Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Preparative Valve-Based Fraction Collector...
  • Page 101 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue...

  • Page 102: Can/Lan Cables

    Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...

  • Page 103: Cables

    Identifying Cables RS-232 Cables RS-232 Cables Description G1530-60600 RS-232 cable, 2 m RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

  • Page 104: Usb

    Identifying Cables To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Preparative Valve-Based Fraction Collector...

  • Page 105: Hardware Information

    Front and Rear View of the Module Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Configuration Switch Settings of the Preparative Valve-Based Fraction Collector This chapter describes the module in more detail on hardware and electronics. Agilent Technologies...

  • Page 106: Electrical Connections

    There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

  • Page 107: Serial Number Information (All)

    Hardware Information Electrical Connections Serial Number Information (ALL) The serial number information on the instrument labels provide the following information: CCXZZ00000 Format Country of manufacturing • DE = Germany • JP = Japan • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module)

  • Page 108: Front And Rear View Of The Module

    Hardware Information Electrical Connections Front and Rear View of the Module Status lamp Fraction ports Inlet port Waste port Figure 10 Front view of the module Power connector External leak sensor connector Power switch Configuration switches CAN ports Regulator adaptor Figure 11 Rear view of the module Preparative Valve-Based Fraction Collector...

  • Page 109: Interfaces

    G7114A/B VWD G7115A DAD G7117A/B/C DAD G7121A/B FLD G7162A/B RID G7165A MWD Fraction Collectors G7159B FC G7166A VFC Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN Card Preparative Valve-Based Fraction Collector...

  • Page 110: Overview Interfaces

    Hardware Information Interfaces Table 14 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI Others G7116A/B MCT Requires a HOST module via CAN G7122A Degasser The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for control via N O T E LAN.
  • Page 111 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 112 Hardware Information Interfaces of all connected modules. Control of analysis is maintained by signal readiness READY for next analysis, followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued.

  • Page 113: Eri (Enhanced Remote Interface)

    Hardware Information Interfaces Special Interfaces There is no special interface for this module. ERI (Enhanced Remote Interface) ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new 1200 Infinity II products using the FUSION core electronics use ERI.
  • Page 114 Hardware Information Interfaces Enhanced Remote IO 1 (START REQUEST) IO 2 (STOP) IO 3 (READY) IO 4 (POWER ON) IO 5 (NOT USED) IO 6 (SHUT DOWN) IO 7 (START) IO 8 (PREPARE) 1 wire DATA DGND +5 V ERI out PGND PGND +24 V ERI out...

  • Page 115: Usb (Universal Serial Bus)

    Hardware Information Interfaces 5V Distribution (Future Use) • Available directly after turn on oft the hosting module (assures that certain base functionality of the device can be detected by firmware). • For digital circuits or similar. • Provided 500 mA maximum. •...

  • Page 116: Configuration Switch Settings Of The Preparative Valve-Based Fraction Collector

    Hardware Information Configuration Switch Settings of the Preparative Valve-Based Fraction Collector Configuration Switch Settings of the Preparative Valve-Based Fraction Collector Configuration Switch Settings Figure 13 Config Switch Figure 14 Dipswitch 1 and 2 Table 16 Configuration Switch Settings Dipswitch 1 Dipswitch 2 Function ON (1)
  • Page 117 Hardware Information Configuration Switch Settings of the Preparative Valve-Based Fraction Collector Special Settings Boot-Resident Firmware update procedures may require this mode in case of firmware loading errors (main firmware part). If you use the following switch settings and power the instrument up again, the instrument firmware stays in the resident mode.
  • Page 118 Hardware Information Configuration Switch Settings of the Preparative Valve-Based Fraction Collector Preparative Valve-Based Fraction Collector...

  • Page 119: Appendix

    Do Not Modify the Instrument In Case of Damage Solvents Compressed Gas Information Safety Symbols Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Agilent Technologies on Internet This chapter provides additional information on safety, legal, and web. Agilent Technologies...

  • Page 120: General Safety Information

    Appendix General Safety Information General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.

  • Page 121: Before Applying Power

    Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔...

  • Page 122: Do Not Operate In An Explosive Atmosphere

    Appendix General Safety Information Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WA R N I N G Explosion hazard ➔ Do not operate the instrument in the presence of flammable gases or fumes. Do Not Remove the Instrument Cover Instrument covers removed WA R N I N G Electrical shock...

  • Page 123: Solvents

    Appendix General Safety Information Solvents Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

  • Page 124: Compressed Gas Information

    Operation of an Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector (G7166A) involves the use of compressed gases. Careless, improper or unskilled use of this instrument can cause injury to personnel, and/or damage to equipment and property.

  • Page 125: Safety Symbols

    Appendix General Safety Information Safety Symbols Table 17 Symbols The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages.
  • Page 126 Appendix General Safety Information Table 17 Symbols Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.

  • Page 127: Waste Electrical And Electronic Equipment Directive

    Appendix Waste Electrical and Electronic Equipment Directive Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005.

  • Page 128: Radio Interference

    Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or...

  • Page 129: Sound Emission

    Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •...

  • Page 130: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Preparative Valve-Based Fraction Collector...
  • Page 131 Index Index dimensions Infinity II internet Agilent Lab Advisor software Agilent Lab Advisor electrical connections Agilent descriptions of on internet electronic waste cable ambient non-operating temperature error message leak sensor open ambient operating temperature position cluster partner missing leak sensor short analog signal pressure cluster partner missing leak...
  • Page 132 Index physical specifications power consideration power consumption power cords voltage range radio interference remote (ERI) waste remote electrical and electronic cables equipment RS-232C WEEE directive cable weight safety class I safety general information standards symbols serial number information shutdown site requirements power cords sound emission special interfaces...
  • Page 133 Index Preparative Valve-Based Fraction Collector...
  • Page 134 • using the module, • troubleshooting and diagnostics, • errors, • maintenance and repair, • parts and materials, • hardware information, • safety and related information. © Agilent Technologies 2016-2018 Printed in Germany 03/2018 *G7166-90000* *G7166-90000* G7166-90000 Rev. B Agilent Technologies...

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