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Agilent Technologies 1260 Infinity User Manual

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  • Page 1 Agilent 1260 Infinity Thermostatted Column Compartment User Manual Agilent Technologies...
  • Page 2 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent For Research Use Only Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1260 Infinity TCC User Manual...
  • Page 3 In This Guide… In This Guide… This manual covers the Agilent 1260 Infinity Thermostatted Column Compartments (G1316A TCC). 1 Introduction to the Column Compartment This chapter gives an introduction to the TCC, instrument overview and internal connectors. 2 Site Requirements and Specifications This chapter provides information about site requirements and specifications for the module.
  • Page 4 9 Parts for Maintenance This chapter provides information on parts for maintenance. 10 Identifying Cables This chapter provides information on cables used with the 1260 Infinity series of HPLC modules. 11 Appendix This chapter provides addition information on safety, legal and web.

  • Page 5: Table Of Contents

    4 How to optimize the Column Compartment Optimizing the Performance of your Column Compartment 5 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators Available Tests depending on User Interfaces Agilent Lab Advisor Software Agilent 1260 Infinity TCC User Manual...
  • Page 6 Micro Column Switching Valve 2 Position-6 Port Micro Column Switching Valve 2 Position/10 Port Capillary Kit Column Regeneration Column Regeneration Kit Accessory Kits 10 Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables External Contact Cable Agilent 1260 Infinity TCC User Manual...
  • Page 7 Agilent Module to PC Agilent 1200 Module to Printer 11 Appendix Safety Symbols The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Lithium Batteries Information Radio Interference Sound Emission Use of Solvents Agilent Technologies on Internet Agilent 1260 Infinity TCC User Manual...
  • Page 8 Contents Agilent 1260 Infinity TCC User Manual...

  • Page 9: Introduction To The Column Compartment

    Agilent 1260 Infinity TCC User Manual Introduction to the Column Compartment Main Features System Overview Column-Identification System Column Switching Valve (Optional) Instrument Layout Electrical Connections Serial Number Information 1260 Infinity Electrical Connections Interfaces Overview Interfaces Setting the 8-bit Configuration Switch (without On-Board LAN)

  • Page 10: Main Features

    Introduction to the Column Compartment Main Features Main Features The Agilent 1260 Infinity Thermostatted Column Compartment is a stackable temperature-controlled column compartment for LC. It is used for heating and cooling to meet extreme requirements of retention time reproducibility. The main features are: •...

  • Page 11: System Overview

    This depends on several factors: flow rate, setpoint temperature, ambient temperature and column dimensions. The higher the flow rate, the faster the column equilibrates (due to thermostatted mobile phase). Agilent 1260 Infinity TCC User Manual...
  • Page 12 Stability of the pressure signal is a good indication for equilibrium. Figure 1 Equilibration of Heat Exchanger and Column Temperature The temperature calibration and verification is described in the Service Manual. Agilent 1260 Infinity TCC User Manual...

  • Page 13: Column-Identification System

    Introduction to the Column Compartment Column-Identification System Column-Identification System The Agilent 1260 Infinity Thermostatted Column Compartment is equipped with a column-identification system. It allows to read and write column-specific information to and from the column-identification tag. Figure 2 Column-Identification System Table 1 on page 13 shows the information that can be stored.
  • Page 14 For example, if the left column is selected, the right column is not updated, and vice versa. If no column switching valve is installed both sides are updated at the same time. Agilent 1260 Infinity TCC User Manual...

  • Page 15: Column Switching Valve (Optional)

    Before switching the valve, switch off the pump or set the flow to zero. Keeping the flow on N O T E while the valve is switched can cause exceeding the maximum pressure. This will stop method or sequence execution. Agilent 1260 Infinity TCC User Manual...
  • Page 16 The sample is injected into series-connected precolumn and analytical column. After the valve has switched, the analytical column flow continues in normal direction. Only the precolumn is back-flushed, eluting highly retained peaks directly to the detector. Agilent 1260 Infinity TCC User Manual...
  • Page 17 Introduction to the Column Compartment Column Switching Valve (Optional) Figure 6 Precolumn Back-flushing Agilent 1260 Infinity TCC User Manual...

  • Page 18: Instrument Layout

    • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent 1260 Infinity TCC User Manual...

  • Page 19: Electrical Connections

    There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.
  • Page 20 00000 Serial number Figure 7 Rear View of the Thermostatted Column Compartment The GPIB interface has been removed with the introduction of the 1260 Infinity modules. N O T E Agilent 1260 Infinity TCC User Manual...

  • Page 21: Interfaces

    THERMOSTAT for G1364C FC-AS G1330B μ CAN-DC- OUT for CAN G1364D FC- slaves G1367E HiP ALS G1377A HiP micro ALS G2258A DL ALS G4226A ALS Detectors G1314B VWD VL G1314C VWD VL+ G1314E/F VWD Agilent 1260 Infinity TCC User Manual...
  • Page 22 • CAN connectors as interface to other modules • LAN connector as interface to the control software • RS-232C as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent 1260 Infinity TCC User Manual...
  • Page 23 • one start bit and one stop bit are always used (not selectable). The RS-232C is designed as DCE (data communication equipment) with a 9-pin male SUB-D type connector. The pins are defined as: Agilent 1260 Infinity TCC User Manual...
  • Page 24 RS-232C Connection Table Direction Function Ground Figure 8 RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. Agilent 1260 Infinity TCC User Manual...
  • Page 25 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 26 START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler. Special Interfaces Some modules have module specific interfaces/connectors. They are described in the module documentation. Agilent 1260 Infinity TCC User Manual...

  • Page 27: Setting The 8-Bit Configuration Switch (Without On-Board Lan)

    • for boot/test modes DIPS 1+2 must be UP plus required mode Switch settings provide configuration parameters for GPIB address, serial communication protocol and instrument specific initialization procedures. With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. N O T E The preferred communication is LAN.
  • Page 28 Parity Use the following tables for selecting the setting which you want to use for RS-232C communication. The number 0 means that the switch is down and 1 means that the switch is up. Agilent 1260 Infinity TCC User Manual...
  • Page 29 Parity No Parity Odd Parity Even Parity One start bit and one stop bit are always used (not selectable). Per default, the module will turn into 19200 baud, 8 data bit with no parity. Agilent 1260 Infinity TCC User Manual...
  • Page 30 Exceptions are diagnosis and repair log books which will not be erased. ➔ Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, a forced cold start has been completed. Agilent 1260 Infinity TCC User Manual...
  • Page 31 Introduction to the Column Compartment Setting the 8-bit Configuration Switch (without On-Board LAN) Table 11 Forced Cold Start Settings (without on-board LAN) Mode Select TEST/BOOT No LAN TEST/BOOT Agilent 1260 Infinity TCC User Manual...
  • Page 32 Introduction to the Column Compartment Setting the 8-bit Configuration Switch (without On-Board LAN) Agilent 1260 Infinity TCC User Manual...

  • Page 33: Site Requirements And Specifications

    Agilent 1260 Infinity TCC User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information about site requirements and specifications for the module. Agilent Technologies...

  • Page 34: Site Requirements

    ➔ Always unplug the power cable before opening the cover. ➔ Do not connect the power cable to the instrument while the covers are removed. Agilent 1260 Infinity TCC User Manual...
  • Page 35 Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
  • Page 36 Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment. ➔ Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. Bench Space The module dimensions and weight (see...

  • Page 37: Physical Specifications

    Operating Altitude Up to 2000 m (6562 ft) Non-operating altitude Up to 4600 m (15091 ft) For storing the module Safety standards: IEC, CSA, Installation Category II, Pollution Degree 2 For indoor use only. Agilent 1260 Infinity TCC User Manual...

  • Page 38: Performance Specifications

    13 Housing All materials recyclable All specifications are valid for distilled water at ambient temperature (25 °C), set point at N O T E 40 °C and a flow range from 0.2 –5 mL/min. Agilent 1260 Infinity TCC User Manual...

  • Page 39: Installing The Column Compartment

    Agilent 1260 Infinity TCC User Manual Installing the Column Compartment Unpacking the Column Compartment Delivery Checklist Optimizing the Stack Configuration One Stack Configuration Two Stack Configuration Installing the Column Compartment Flow Connections of the Column Compartment Placing Columns Column-Identification Tag...

  • Page 40: Unpacking The Column Compartment

    Unpacking the Column Compartment If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C A U T I O N If there are signs of damage, please do not attempt to install the module.

  • Page 41: Optimizing The Stack Configuration

    Infinity LC in the following configuration (see Figure 10 on page 42 and Figure 11 on page 43). This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required. Agilent 1260 Infinity TCC User Manual...
  • Page 42 Installing the Column Compartment Optimizing the Stack Configuration Figure 10 Recommended Stack Configuration (Front View) Agilent 1260 Infinity TCC User Manual...
  • Page 43 Installing the Column Compartment Optimizing the Stack Configuration Figure 11 Recommended Stack Configuration (Rear View) Agilent 1260 Infinity TCC User Manual...

  • Page 44: Two Stack Configuration

    A slightly longer capillary is required between the pump and autosampler. See Figure 12 on page 44 and Figure 13 on page 45. Figure 12 Two stack configuration (front view) Agilent 1260 Infinity TCC User Manual...
  • Page 45 Installing the Column Compartment Optimizing the Stack Configuration Figure 13 Two stack configuration (rear view) Agilent 1260 Infinity TCC User Manual...

  • Page 46: Installing The Column Compartment

    Never plug the power line back in when cover is removed. 1 Place the column compartment in the stack or on the bench in a horizontal position. Agilent 1260 Infinity TCC User Manual...
  • Page 47 3 Connect the power cable to the power connector at the rear of the column compartment. 4 Connect the CAN cable to other Agilent 1260 Infinity modules. 5 If Agilent ChemStation is the controller, connect the LAN connection to the LAN interface board in the detector.
  • Page 48 The column compartment is turned on when the line power switch is pressed and the green N O T E indicator lamp is illuminated. The column compartment is turned off when the line power switch is protruding and the green light is OFF. Agilent 1260 Infinity TCC User Manual...

  • Page 49: Flow Connections Of The Column Compartment

    ➔ The amount of substances should be reduced to the minimal volume required for the analysis. ➔ Do not operate the instrument in an explosive atmosphere. Agilent 1260 Infinity TCC User Manual...
  • Page 50 For more information on column identification, see The internal volumes of the heat exchanger assemblies “Column-Identification System” on page 13 . comprise a volume of 3 µl (left) and 6 µl (right). The internal capillary diameter is 0.17 mm. Agilent 1260 Infinity TCC User Manual...
  • Page 51 Fix the column with the column clip from the accessory N O T E kit. “Column Switching Valve (Optional)” on page 15 on how to connect the column selection valve. Agilent 1260 Infinity TCC User Manual...
  • Page 52 The installation of the column compartment has now been completed. Always operate the TCC with the front cover in place for proper thermostatting conditions N O T E and to protect the column area against strong drafts from the ouside. Agilent 1260 Infinity TCC User Manual...

  • Page 53: Placing Columns

    Figure 16 on page 53 and Figure 17 on page 53. The Agilent logo should always be at front. Figure 16 Column-Identification Tag for Left Heat Exchanger Figure 17 Column-Identification Tag for Right Heat Exchanger Agilent 1260 Infinity TCC User Manual...

  • Page 54: Column Clip

    Installing the Column Compartment Placing Columns Column Clip For better positioning of the column on the heat exchanger a column clip is available (see “Accessory Kits” on page 115). Figure 18 Column clip Agilent 1260 Infinity TCC User Manual...

  • Page 55: How To Optimize The Column Compartment

    Agilent 1260 Infinity TCC User Manual How to optimize the Column Compartment Optimizing the Performance of your Column Compartment This chapter provides information on how to optimize the thermostatted column compartement. Agilent Technologies...

  • Page 56: Optimizing The Performance Of Your Column Compartment

    • Keep the left and right heat exchanger temperature the same unless you do specific applications. • Assure that the front cover is always closed. Agilent 1260 Infinity TCC User Manual...

  • Page 57: Troubleshooting And Diagnostics

    Agilent 1260 Infinity TCC User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators Power Supply Indicator Module Status Indicator Available Tests depending on User Interfaces Agilent Lab Advisor Software Overview about the troubleshooting and diagnostic features.

  • Page 58: Overview Of The Module's Indicators And Test Functions

    Normally, temperature calibration is not required throughout the lifetime of the instrument. However, in order to comply with local regulatory requirements, calibration and verification may be required. The following sections describe these functions in detail. Agilent 1260 Infinity TCC User Manual...

  • Page 59: Status Indicators

    Figure 19 Location of Status indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON. Agilent 1260 Infinity TCC User Manual...

  • Page 60: Module Status Indicator

    • A fast red-blinking (modules with on-board LAN) or fast yellow-blinking (modules without on-board LAN) indicator indicates that the module is in boot loader mode (e.g. during update of main firmware). In such a case try to re-boot the module or try a cold-start. Agilent 1260 Infinity TCC User Manual...

  • Page 61: Available Tests Depending On User Interfaces

    Preferred tool should be the Agilent Lab Advisor Software, see “Agilent Lab Advisor Software” on page 62. Table 15 Test Functions available vs. User Interface - TCC Test Lab Advisor Software Agilent ChemStation Instant Pilot G4208A Thermostat Function Test Temperature Calibration section Maintenance Agilent 1260 Infinity TCC User Manual...

  • Page 62: Agilent Lab Advisor Software

    Agilent Lab Advisor software may differ from the descriptions in this manual. For details refer to the Agilent Lab Advisor software help files. This manual provides lists with the names of Error Messages, Not Ready messages, and other common issues. Agilent 1260 Infinity TCC User Manual...

  • Page 63: Error Information

    Agilent 1260 Infinity TCC User Manual Error Information What Are Error Messages General Error Messages Timeout Shut-Down Remote Timeout Synchronization Lost Leak Leak Sensor Open Leak Sensor Short Compensation Sensor Open Compensation Sensor Short TCC Error Messages Left Fan Failed...

  • Page 64: What Are Error Messages

    (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the red status indicator at the front of the module is switched on, and an entry is written into the module logbook. Agilent 1260 Infinity TCC User Manual...

  • Page 65: General Error Messages

    An external instrument has generated a shut-down signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message. Agilent 1260 Infinity TCC User Manual...

  • Page 66: Remote Timeout

    Exchange the remote cable. Defective remote cable. Check the instrument for defects (refer to the Defective components in the instrument instrument’s documentation). showing the not-ready condition. Agilent 1260 Infinity TCC User Manual...

  • Page 67: Synchronization Lost

    TCC board. Probable cause Suggested actions Use a higher temperature setpoint. Condensation. Ensure all fittings are tight. Loose column fittings. Exchange defective capillaries. Broken capillary. Exchange the valve seal. Leaking column-switching valve seal. Agilent 1260 Infinity TCC User Manual...

  • Page 68: Leak Sensor Open

    Probable cause Suggested actions Please contact your Agilent service Defective flow sensor. representative. Please contact your Agilent service Leak sensor incorrectly routed, being representative. pinched by a metal component. Agilent 1260 Infinity TCC User Manual...

  • Page 69: Compensation Sensor Open

    If the resistance across the sensor falls below the lower limit, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective main board. representative. Agilent 1260 Infinity TCC User Manual...

  • Page 70: Tcc Error Messages

    5 seconds, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Fan cable disconnected. representative. Please contact your Agilent service Defective fan. representative. Please contact your Agilent service Defective TCC board. representative. Agilent 1260 Infinity TCC User Manual...

  • Page 71: Right Fan Failed

    OFF, and the error message is generated. Probable cause Suggested actions The top foam was removed during operation. Please contact your Agilent service representative. Please contact your Agilent service Foam not activating the sensor. representative. Agilent 1260 Infinity TCC User Manual...

  • Page 72: Cover Violation

    The temperature of the left heat exchanger did not reach the temperature setpoint within the timeout threshold. Probable cause Suggested actions Please contact your Agilent service Defective left heater assembly. representative. Please contact your Agilent service Defective TCC board. representative. Agilent 1260 Infinity TCC User Manual...

  • Page 73: Right Temperature Timeout

    Flex board not connected (only if all left or representative. right sensor error messages appear simultaneously). Please contact your Agilent service Defective heater assembly. representative. Please contact your Agilent service Defective TCC board. representative. Agilent 1260 Infinity TCC User Manual...

  • Page 74: Heater Profile

    Probable cause Suggested actions Please contact your Agilent service Defective column-switching valve. representative. Please contact your Agilent service Defective TCC board. representative. Agilent 1260 Infinity TCC User Manual...

  • Page 75: Column Temperature

    105 °C, and the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective heater assembly. representative. Please contact your Agilent service Defective TCC board. representative. Agilent 1260 Infinity TCC User Manual...

  • Page 76: Heatsink Temperature

    The processor checks the function of the heater circuits continually. If a defect is detected in the control circuit, the processor switches OFF the heater (peltier) assemblies, and the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective TCC board. representative. Agilent 1260 Infinity TCC User Manual...

  • Page 77: Test Functions

    Agilent 1260 Infinity TCC User Manual Test Functions Thermostat Function Test Evaluating the Thermostat Function Test Pressure Test Column Thermostat Temperature Calibration Temperature Calibration Procedure Column Thermostat Calibration Problems Installing the Temperature Sensor This chapter describes the TCC’s built in test functions.

  • Page 78: Thermostat Function Test

    The time required to reach 30 °C is a measure of heating efficiency. Thermostat Function Test Result A typical Thermostat Function Test profile is shown in Figure 20 on page 78. Figure 20 Typical Thermostat Function Test Profile Agilent 1260 Infinity TCC User Manual...
  • Page 79 Test Functions Thermostat Function Test Thermostat Test with Agilent LabAdvisor 1 1. Select the Thermostat Test and start the test. Figure 21 Thermostat Test width = 12.5 cm Agilent 1260 Infinity TCC User Manual...

  • Page 80: Evaluating The Thermostat Function Test

    Poor peltier efficiency (if setpoint temperatures can still be reached, and are stable, there is no requirement to exchange the heater assembly). Exchange the heater assembly. Defective sensors on flex board. Exchange the heater assembly. Defective heater assembly. Agilent 1260 Infinity TCC User Manual...

  • Page 81: Pressure Test

    Do not use the test for modules having a lower maximum pressure than the pump as this will damage the valve. For example do not use 400 bar valve in a TCC in combination with a 600 bar pump (G1312B Binary Pump SL). Agilent 1260 Infinity TCC User Manual...

  • Page 82: Column Thermostat Temperature Calibration

    The column thermostat is calibrated correctly when the measured temperature (using the external measuring device, “Temperature Calibration Procedure” on page 83) and the cross-over temperature (36 °C) of both heat exchangers (left and right) are within a range of ± 0.5 °C. Agilent 1260 Infinity TCC User Manual...

  • Page 83: Temperature Calibration Procedure

    For the measuring and calibration process Agilent Technologies recommends a N O T E thermometer with 0.1 °C precision. Contact the local Agilent Technologies support representative for ordering information. The figures in this procedure refer to a specific type of temperature sensor (Heraeus N O T E Quat340, quartz surface-temperature measurement sensor).
  • Page 84 Figure 23 Temperature Calibration - Step 1 (Start) width=12.5 cm 2 Wait for the temperature to stabilize at the calibration temperature (36 °C). Figure 24 Temperature Calibration - Step 2 (Wait for stabilization) width=12.5 cm Agilent 1260 Infinity TCC User Manual...
  • Page 85 Figure 26 OnePoint Calibration - Step 4 (Calibration done) width=12.5 cm 5 Repeat the calibration procedure for the right heat exchanger. Agilent 1260 Infinity TCC User Manual...
  • Page 86 In case the instrument must be converted back to standard 1-point calibration, a special command has to used, see “Turning off the 2-point calibration” page 88. The figures below show the calibration process with the Agilent LabAdvisor software. It is similar to the 1-Point Calibration. Agilent 1260 Infinity TCC User Manual...
  • Page 87 Test Functions Column Thermostat Temperature Calibration Figure 27 Two Point Calibration – Start width = 12.5 cm Figure 28 Two Point Calibration – Define the two temperatures width=12.5 cm Agilent 1260 Infinity TCC User Manual...
  • Page 88 2 For right heat exchanger use the command E2PC 1,0 and Execute. RA 0000 E2PC 1,0 The user interface will reply with if command was entered correctly. 3 Perform the “Temperature Calibration” on page xx. Agilent 1260 Infinity TCC User Manual...

  • Page 89: Column Thermostat Calibration Problems

    Hardware Failures Probable hardware failures that can lead to a failed calibration procedure are: • Defective or wrongly calibrated measuring device. • Defective heater assembly. • Defective ambient-temperature sensor. • Defective CCM board. Agilent 1260 Infinity TCC User Manual...

  • Page 90: Installing The Temperature Sensor

    Other sensors may require a different fixing. Remove the front cover. Install the temperature sensor at the measurement position on the left heat exchanger. Route the sensor wire through the slit in the leak tray. Re-install the front cover. Agilent 1260 Infinity TCC User Manual...

  • Page 91: Maintenance

    Agilent 1260 Infinity TCC User Manual Maintenance Warnings and Cautions Introduction to Maintenance Overview of Maintenance Cleaning the Module Changing Column Identification Tags Replacing Head Parts of Column Switching Valve Correcting Leaks Replacing the Module’s Firmware This chapter describes the maintenance and repair of the TCC.

  • Page 92: Warnings And Cautions

    Sharp metal edges WA R N I N G Sharp-edged parts of the equipment may cause injuries. ➔ To prevent personal injury, be careful when getting in contact with sharp metal areas. Agilent 1260 Infinity TCC User Manual...
  • Page 93 C A U T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. Agilent 1260 Infinity TCC User Manual...

  • Page 94: Introduction To Maintenance

    Introduction to Maintenance Introduction to Maintenance shows the main user accessible assemblies of the Agilent 1260 Infinity Thermostatted Column Compartment. These parts can be accessed from the front (simple repairs) and don't require to remove the TCC from the system stack.

  • Page 95: Overview Of Maintenance

    “Replacing Head Parts of If the valve performance shows Column Switching Valve” indication of leakage or wear page 99 “Correcting Leaks” on page 102 If a leak has occurred Check for leaks Agilent 1260 Infinity TCC User Manual...

  • Page 96: Cleaning The Module

    WA R N I N G Liquid in the module electronics can cause shock hazard and damage the module. ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any fittings. Agilent 1260 Infinity TCC User Manual...

  • Page 97: Changing Column Identification Tags

    53. The Agilent logo should always be at front. When correctly placed on the heat exchanger, the distance between tag and antenna is 1–2 mm. This is the optimum distance for proper function. Agilent 1260 Infinity TCC User Manual...
  • Page 98 Column-Identification Tag for Right Heat Exchanger 3 For columns with small diameter, a cable tie wrap should be used to fix the column identification tag to the column. Assure that the tie wrap does not block the front cover. Agilent 1260 Infinity TCC User Manual...

  • Page 99: Replacing Head Parts Of Column Switching Valve

    Replacing Head Parts of Column Switching Valve Figure 33 Column Switching Valve Parts When If valve leaks. Tools required 1/4 inch wrench 9/64 inch hex key Parts required Description For parts refer to “Valve Options Overview” on page 106. Agilent 1260 Infinity TCC User Manual...
  • Page 100 8 Replace the stator ring. Ensure the stator ring is flush with the valve body. 9 Place the new (if required) ceramic stator face in place on the stator head. Reinstall the stator head. Agilent 1260 Infinity TCC User Manual...
  • Page 101 For example do not use 400 bar valve in a TCC in combination with a 600 bar pump (G1312B Binary Pump SL). 12 Perform a Pressure Test to ensure the valve is pressure tight to 400 bar. Agilent 1260 Infinity TCC User Manual...

  • Page 102: Correcting Leaks

    2 Use a pipette and tissue to dry the leak sensor area. 3 Observe the capillary connections and the column switching valve for leaks and correct, if required. 4 Re-install the front cover. Figure 34 Possible Leak Areas Agilent 1260 Infinity TCC User Manual...

  • Page 103: Replacing The Module's Firmware

    2 To load the firmware into the module follow the instructions in the documentation. Module Specific Information Table 17 Module Specific Information (G1316A/B) G1316A Initial firmware (main and resident) Depends on main board revision. Newer versions (G1316-66530 and higher) allow A.05.05 and above only. Agilent 1260 Infinity TCC User Manual...
  • Page 104 Maintenance Replacing the Module’s Firmware Table 17 Module Specific Information (G1316A/B) G1316A Compatibility with 1100 / 1200 series modules always Conversion to / emulation Agilent 1260 Infinity TCC User Manual...

  • Page 105: Parts For Maintenance

    Agilent 1260 Infinity TCC User Manual Parts for Maintenance Valve Options Overview Column Switching Valve 2 Position/6 Port Micro Column Switching Valve 2 Position-6 Port Micro Column Switching Valve 2 Position/10 Port Capillary Kit Column Regeneration Column Regeneration Kit Accessory Kits...

  • Page 106: Valve Options Overview

    Rhebuild kit for 7750-030 valve (p/n 0101-1258) includes 3-groove rotor seal, stator face assy, isolation seal, instructions. re-build kit Rebuild kit, includes PEEK rotor seal, PEEK stator face, hex key (p/n 0101-1360) in- cludes PEEK rotor seal, PEEK stator face, hex key. Agilent 1260 Infinity TCC User Manual...

  • Page 107: Column Switching Valve 2 Position/6 Port

    Column Switching Valve 2 Position/6 Port Column Switching Valve 2 Position/6 Port Agilent 1260 Infinity and 1200 Series 1316A modules with serial numbers DE90373728 and N O T E above use parts from the G1316C TCC. For replacements use NEW part numbers below.
  • Page 108 Parts for Maintenance Column Switching Valve 2 Position/6 Port Figure 35 Column Switching Valve Parts Agilent 1260 Infinity TCC User Manual...

  • Page 109: Micro Column Switching Valve 2 Position-6 Port

    Micro Column Switching Valve 2 Position-6 Port Description G1316-67006 Valve Kit MICRO 2POS/6PORT 400 bar (incudues valve, Valve Liner and Upgrade Note) 0100-2089 Stator face 0100-2087 Rotor seal 3 grooves, Vespel 1535-4045 Bearing Ring, (Qty 1, replacement) Agilent 1260 Infinity TCC User Manual...

  • Page 110: Micro Column Switching Valve 2 Position/10 Port

    Valve Kit MICRO 2POS/10PORT 400 bar (incudues valve, Valve Liner and Upgrade Note) 0101-1360 Rebuild kit, includes PEEK rotor seal, PEEK stator face, hex key 0100-1852 Isolation seal 0101-1362 Stator face G1316-68711 Capillary kit Agilent 1260 Infinity TCC User Manual...

  • Page 111: Capillary Kit Column Regeneration

    Capillary (p/n Column 2 Valve (port 8) 0.17 for long 5021-1816) column Capillary (p/n Column 2 Valve (port 8) 0.17 for short 5065-9931) column Capillary (p/n Valve (port 5) Valve (port 10) 0.17 5021-1816) Agilent 1260 Infinity TCC User Manual...
  • Page 112 Ferrule back 1/16" SST, qty=2, re-order pack of 10 0890-1763 PEEK tubing 1/16” i.d. 0.18 mm, 1500 mm lg 8710-1930 Plastic tubing cutter 8710-2462 Hex Key Driver 3/32 inch 8710-2391 Rheotool socket wrench ¼ inch Agilent 1260 Infinity TCC User Manual...

  • Page 113: Column Regeneration Kit

    Seat Capillary, 100 mm, 0.12 mm i.d. (pack of 2) Seat capillary (p/n G1367-87303) PEEK fitting, special for Chip-LC PEEK fitting (p/n G4240-43200) Flexible PEEK Tubing, 450 mm, 0.4 mm i.d. valve to waste Flexible PEEK tubing (p/n 5022-6503) Agilent 1260 Infinity TCC User Manual...
  • Page 114 Parts for Maintenance Column Regeneration Kit Figure 36 Connection Diagram for Column Regeneration Agilent 1260 Infinity TCC User Manual...

  • Page 115: Accessory Kits

    Wrench open end, 5/16 – 3/8 inch G1375-87309 Fused silica/PEEK capillary 50 µm, 280 mm (4x) 5181-1516 CAN cable, Agilent module to module, 0.5 m 5022-2186 Micro Valve Fitting (2x) 5001-3702 2 Column holders for µ-LC columns Agilent 1260 Infinity TCC User Manual...
  • Page 116 Parts for Maintenance Accessory Kits Agilent 1260 Infinity TCC User Manual...

  • Page 117: Identifying Cables

    Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables External Contact Cable Agilent Module to PC Agilent 1200 Module to Printer This chapter provides information on cables used with the 1260 Infinity series of HPLC modules. Agilent Technologies...

  • Page 118: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent module to 3394/6 integrators...
  • Page 119 It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1260 Infinity TCC User Manual...

  • Page 120: Analog Cables

    The other end depends on the instrument to which connection is being made. Agilent Module to 3394/6 Integrators p/n 35900-60750 Pin 3394/6 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + Agilent 1260 Infinity TCC User Manual...
  • Page 121 Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin 3394/6 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent 1260 Infinity TCC User Manual...

  • Page 122: Remote Cables

    Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to 3396A Integrators...
  • Page 123 Not connected 6 - Yellow 6 - Yellow Power on High 7 - Red 7 - Red Ready High 8 - Green 8 - Green Stop 9 - Black 9 - Black Start request Agilent 1260 Infinity TCC User Manual...
  • Page 124 3 - Gray Start 4 - Blue Shut down 5 - Pink Not connected 6 - Yellow Power on High 7 - Red Ready High 8 - Green Stop 9 - Black Start request Agilent 1260 Infinity TCC User Manual...

  • Page 125: Bcd Cables

    Black BCD 0 Orange BCD 3 BCD 2 Brown BCD 1 Gray Digital ground Gray Gray/pink BCD 11 Red/blue BCD 10 White/green BCD 9 Brown/green BCD 8 not connected not connected + 5 V Agilent 1260 Infinity TCC User Manual...
  • Page 126 Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Agilent 1260 Infinity TCC User Manual...

  • Page 127: Can/Lan Cables

    CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent 1260 Infinity TCC User Manual...

  • Page 128: External Contact Cable

    EXT 2 Grey EXT 3 Pink EXT 3 Blue EXT 4 EXT 4 Black Not connected Violet Not connected Grey/pink Not connected Red/blue Not connected White/green Not connected Brown/green Not connected White/yellow Not connected Agilent 1260 Infinity TCC User Manual...

  • Page 129: Agilent Module To Pc

    It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1260 Infinity TCC User Manual...

  • Page 130: Agilent 1200 Module To Printer

    Agilent 1200 Module to Printer Description 5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module. Agilent 1260 Infinity TCC User Manual...

  • Page 131: Appendix

    Agilent 1260 Infinity TCC User Manual Appendix Safety Symbols The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Lithium Batteries Information Radio Interference Sound Emission Use of Solvents Agilent Technologies on Internet This chapter provides addition information on safety, legal and web.

  • Page 132: Safety Symbols

    C A U T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1260 Infinity TCC User Manual...
  • Page 133 Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Agilent 1260 Infinity TCC User Manual...
  • Page 134 When working with solvents please observe appropriate safety procedures (e.g. goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. Agilent 1260 Infinity TCC User Manual...

  • Page 135: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)

    “Monitoring and Control instrumentation” product. Do not dispose off in domestic household waste To return unwanted products, contact your local Agilent office, or see www.agilent.com for more information. Agilent 1260 Infinity TCC User Manual...

  • Page 136: Lithium Batteries Information

    Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten. ➔ Brukt batteri returneres appararleverandoren. Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien N O T E maar inleveren als KCA. Agilent 1260 Infinity TCC User Manual...

  • Page 137: Radio Interference

    If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. Agilent 1260 Infinity TCC User Manual...

  • Page 138: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 1260 Infinity TCC User Manual...

  • Page 139: Use Of Solvents

    THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides, • Solvents containing strong complexing agents (e.g. EDTA), • Mixtures of carbon tetrachloride with 2-propanol or THF. Agilent 1260 Infinity TCC User Manual...

  • Page 140: Agilent Technologies On Internet

    Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the modules for download. Agilent 1260 Infinity TCC User Manual...
  • Page 141 Communication settings leak cable RS-232C left fan failed analog compensation sensor open left temperature timeout compensation sensor short open cover condensation remote timeout external contact Configuration right fan failed two stack Agilent 1260 Infinity TCC User Manual...
  • Page 142 I installation open cover bench space safety information operating Altitude capillaries and waste tubings lithium batteries operating temperature column safety optimization flow connections general information optimizing the performance module standards temperature sensor symbols Agilent 1260 Infinity TCC User Manual...
  • Page 143 Agilent 1260 Infinity TCC User Manual...
  • Page 144 In This Book This manual contains technical reference information about the Agilent 1260 Infinity Thermostatted Column Compartment (G1316A TCC). The manual describes the following: • introduction and specifications, • installation, • using and optimizing, • troubleshooting and diagnose, • maintenance, •...

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