Agilent Technologies 1220 Infinity II LC User Manual page 133

Figure 45
Figure 46
In a four-component mixture, only biphenyl was recorded. The other three
peaks were suppressed because they did not meet the ratio-qualifier criterion
and therefore the output was set to zero. The characteristic wavelengths
249 nm (λ
on page 133. The ratio range was set at 2 – 2.4 (2.2 ±10%). Only when the ratio
between 249 and 224 nm was within this range, is the signal plotted. Of all
four peaks, only the third fulfilled the criterion
others were not plotted.
1220 Infinity II LC System User Manual
Agilent 1220 Infinity II LC Diode Array Detector (DAD)
Wavelength Selection for Ratio Qualifiers
Biphenyl
Time (min)
Selectivity by Ratio Qualifiers
) and 224 nm (λ ) were found from the spectra shown in
1 2
Signals at 250 nm
o-Terphenyl
No selectivity
With ratio qualifier
249/224 nm = 3.520%
(Figure 46
Detector Description
Figure 45
on page 133). The
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