Agilent Technologies 1220 Infinity II LC User Manual page 179

Holmium Oxide Test
The holmium oxide test uses characteristic absorbance maxima of the built-in
holmium oxide filter to verify wavelength accuracy (see also
Verification and Recalibration"
1-nm slit is moved into the light path automatically. To eliminate effects due to
absorbing solvents, the test should be done with water in the flow cell or with
removed flow cell.
See also
N O T E
Holmium Oxide Test Evaluation
Limits:
361.0 nm
453.7 nm
536.7 nm
The test is evaluated by the instrument, and the measured maxima are
displayed automatically. The test fails if one or more of the maxima lies
outside of the limits (see
1220 Infinity II LC System User Manual
"Declaration of Conformity for HOX2 Filter"
360.0 - 362.0 nm (± 1nm)
452.7 - 454.7 nm (± 1nm)
535.7 - 537.7 nm (± 1nm)
Figure 55
Test Functions and Calibration
Diode Array Detector (DAD)
on page 188). When the test is started, the
on page 389.
on page 181).
9
"Wavelength
179