Injecting Sample - Agilent Technologies 1220 Infinity II LC User Manual

Injecting Sample

Ejection of mobile phase
WA R N I N G
When using sample loops larger than 100 µL, mobile phase may be ejected from the
needle port as the mobile phase in the sample loop decompresses.
➔
LOAD Position
In the LOAD position (see
directly to the column (ports 4 and 5 connected), and the needle port is
connected to the sample loop. At least 2 to 3 sample-loop volumes (more if
better precision is required) of sample should be injected through the needle
port to provide good precision. The sample fills the loop, and excess sample is
expelled through the vent tube connected to port 2.
Figure 29
1220 Infinity II LC System User Manual
Please observe appropriate safety procedures (for example, goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.
LOAD Position
Figure 29 on page 101), the pump is connected
Injection System Description
Manual Injector
6
101