Agilent Technologies 1220 Infinity II LC User Manual page 93

Optimizing the compressibility compensation setting
The default compressibility compensation setting is 46·10
represents an average value. Under normal conditions, the default setting
reduces the pressure pulsation to values (below 1 % of system pressure) that
are sufficient for most applications and for all gradient analyses. For
applications using sensitive detectors, the compressibility settings can be
optimized by using the values for the various solvents. If the solvent in use is
not listed in the compressibility tables, when using isocratic mixtures of
solvents and if the default settings are not sufficient for your application, the
following procedure can be used to optimize the compressibility settings.
When using mixtures of solvents, it is not possible to calculate the compressibility of the
N O T E
mixture by interpolating the compressibility values of the pure solvents used in that
mixture or by applying any other calculation. In these cases, the following empirical
procedure has to be applied to optimize your compressibility setting.
1 Start the pump with the required flow rate.
2 Before starting the optimization procedure, the flow must be stable. Use
3 Your pump must be connected to control software with which the pressure
1220 Infinity II LC System User Manual
degassed solvent only. Check the tightness of the system with the pressure
test.
and %-ripple can be monitored.
Solvent Delivery System Description
Compressibility Compensation
-6
/bar. This setting
5
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