Run 1: Calibration; Run 2: Unknown Sample - Agilent Technologies 8453 Operator's Manual

5
Quantification
ISTD Calculation

Run 1: Calibration

1 The calibration points are constructed by calculating an amount ratio and a
2 An equation for the curve through the calibration points is calculated using

Run 2: Unknown Sample

1 The response of the compound in the unknown sample is divided by the
2 An amount ratio for the unknown is calculated using the curve fit equation
124
response ratio for each level of a particular peak in the calibration table.
The amount ratio is the amount of the compound divided by the amount of
the internal standard at this level.
The response ratio is the area of the compound divided by the area or
height of the internal standard at this level.
the type of curve fit specified in the Calibration Settings dialog box or
Calibration Curve dialog box.
Figure 29 Amount Ratio
response of the internal standard in the unknown sample to give a response
ratio for the unknown.
determined in step 2 above, and the actual amount of ISTD in the sample.
Understanding Your Agilent ChemStation