Overview; Defining The Initial Baseline - Agilent Technologies 8453 Operator's Manual

Overview

To integrate a chromatogram/electropherogram the integrator:
1 defines the initial baseline,
2 continuously tracks and updates the baseline,
3 identifies the start time for a peak and marks this point with a vertical tick
4 finds the apex of each peak and prints the retention/migration time,
5 identifies the end time for the peak, and marks this point with a vertical
6 constructs a baseline, and
7 calculates the area, height, and peak width for each peak.
This process is controlled by integration events. The most important events
are initial slope sensitivity, peak width, area reject and height reject. The
software allows you to set initial values for these and other events. The initial
values take effect at the beginning of the chromatogram. In addition, the auto
integration function provides a set of initial events that you can optimize
further.
In most cases, the initial events will give good integration results for the entire
chromatogram, but there may be times when you want more control over the
progress of an integration.
The software allows you to control how an integration is performed by
enabling you to program new integration events at appropriate times in the
chromatogram.
For more information, see

Defining the Initial Baseline

Because baseline conditions vary according to the application and detector
hardware, the integrator uses parameters from both the method and the data
file to optimize the baseline.
Understanding Your Agilent ChemStation
mark,
tick mark,
"Initial Events" on page 105.
4
Integration

Overview

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