Agilent Technologies 8453 Operator's Manual page 409

6
Peak Identification
Corrected Retention/Migration Times
progresses. Often during a long run the retention/migration time changes
non-uniformly. In such cases better results are obtained using multiple
reference peaks spaced at intervals across the run. This splits the signal into
separate zones. Within each zone the deviation between retention/migration
times is assumed to change linearly, but the rate of change is determined
separately for each zone.
The time correction algorithm may fail if the retention times of multiple reference peaks are
N O T E
too close to each other and are not distributed across the total run time.
Understanding Your Agilent ChemStation 135